Salinity-imposed changes of some isozymes and total leaf protein expression in five mangroves from two different habitats

Figures & data

Figure 1.  (A) Bar diagram of total leaf proteins with standard errors from the plants grown in saline and non-saline habitat. (B) SDS-PAGE documentation of five taxa grown in saline and non-saline habitat; in the pairs of lanes, the left lane represents saline and the right one forms non-saline habitat plants. M – Protein marker, 1 – B. gymnorrhiza, 2 – E. agallocha, 3 – H. fomes, 4 – P. paludosa and 5 – X. granatum.

Figure 2.  (A–D) Documented native gel electrophoresis. (A) Peroxidase; (B) Superoxide dismutase; (C) Esterase; (D) Acid phosphatase. In all cases, left lane from non-saline and right one from saline plants. 1 – B. gymnorrhiza, 2 – E. agallocha, 3 – H. fomes, 4 – P. paludosa and 5 – X. granatum. (E–H) Bar diagrams of quantitative estimation of enzymes with standard errors bars.

Table 1. Band intensity (OD) and Relative Mobility Fraction (RMF) of isozymes from mangroves in two environments.

	Peroxidase				Superoxide dismutase				Esterase				Acid phosphatase
	Saline		Non-saline		Saline		Non-saline		Saline		Non-saline		Saline		Non-saline
Enzymes Env. Plants	RMF	OD	RMF	OD	RMF	OD	RMF	OD	RMF	OD	RMF	OD	RMF	OD	RMF	OD
B. gymnorrhiza	0.07	163.5	0.09	8.4	0.31	135.0	0.03	16.42	0.37	150.0	0.36	17.33	0.32	148.6	0.53	194
	0.17	121.1	0.18	23.02	0.42	134.0	0.11	25.75	0.48	153.0	0.48	20.0	0.49	157.0
	0.24	99.38	0.47	6.76	0.66	127.0	0.18	41.53	0.53	221.0
	0.35	26.12	0.59	8.96	0.73	142.0
	0.41	24.32	0.68	51.37
	0.5	21.11
	0.67	20.64
	0.79	72.51
E. agallocha	0.07	80.12	0.18	6.03	0.02	129.38	0.18	113.0	0.17	214.0	0.24	102.27	0.40	196.0	0.53	26.15
	0.15	25.3	0.26	8.05	0.31	170.07	0.33	163.0	0.37	212.0	0.3	30.82
	0.21	115.66			0.34	86.36	0.49	162.0	0.53	210.0	0.56	14.62
					0.57	90.99
					0.66	74.39
H. fomes	0.07	166.0	0.18	206.0	0.06	63.0	0.33	5.43	0.37	214.0	0.36	53.6	0.17	127.4	0.19	189.0
	0.17	184.0	0.26	204.0	0.2	26.5	0.63	6.48	0.48	226.0	0.48	32.48	0.36	146.6	0.37	245.0
	0.31	196.0	0.76	182.0	0.31	43.45	0.75	5.42	0.62	205.0			0.49	168.4
					0.42	50.31			0.71	198.5
					0.78	138.7
P. paludosa	0.04	184.94	0.18	154.0	0.06	160.0	0.11	40.0	0.1	178.0	0.3	177.0	0.12	227.0	0.17	96.03
	0.09	125.62	0.26	192.0	0.31	131.0	0.26	42.0	0.37	186.0	0.48	174.0	0.32	195.0	0.46	29.31
	0.41	139.67	0.76	39.0	0.34	128.2	0.39		0.48	222.0			0.4	185.0	0.63	18.6
	0.67	136.81			0.48	176.0							0.49	214.0
					0.78	184.0
X. granatum	0.07	89.91	0.59	168.0	0.14	44.0	0.24	5.5	0.10	190.0	0.3	59.5	0.17	210.0	0.12	203.0
	0.31	18.0	0.68		0.42	104.0	0.49	12.0	0.32	214.0			0.32	194.0	0.37	248.0
	0.41	75.43	0.76	180.0	0.57	41.0	0.63	5.87	0.37	223.0			0.49	247.0	0.46	194.0
				150.0	0.84	155.0			0.48	174.0				210.0
					0.87	147.0			0.71	162.0

Table 2. Correlations among the different enzymes and total proteins in the plants of two habitats.

	B. gymnorrhiza		E. agallocha		H. fomes		P. paludosa		X. granatum
Species Treatment	Ns	S	Ns	S	Ns	S	Ns	S	Ns	S	Treatment
PRO	0.186	−0.571**	0.380	−0.754*	0.045	−0.529*	−0.383	−0.731*	0.145	−0.705*	SOD
	0.110	0.301	0.308	−0.442	0.795**	−0.348	0.698*	0.187	0.555	−0.013	POX
	0.213	0.667*	0.603	−0.66*	0.468	0.099	0.554	−0.517	0.518	−0.363	EST
	−0.206	0.430	−0.510	−0.65*	0.192	−0.9**	−0.254	0.495	−0.407	−0.403	ACP
*Significant at 0.01%; **Significant at 0.05%; Ns, non-saline; S, saline.