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Plant-Microorganism Interactions

Induction of β-1,3-glucanase and chitinase activity in the defense response of Eruca sativa plants against the fungal pathogen Alternaria brassicicola

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Pages 155-161 | Received 29 Jan 2012, Accepted 22 Mar 2012, Published online: 23 Apr 2012

Figures & data

Figure 1.  β-1,3-glucanase activity in control and pathogen (Alternaria brassicicola) inoculated 10 days (A) and one month (B) old arugula (Eruca sativa) plants viz. T-27 and RTM-2002. – • – T-27 control, – ○ – T-27 inoculated, –▾– RTM-2002 control, – ▿ – RTM-2002 inoculated. Each value represents the mean of four replicates with SE determined. Mean differences were significant at ≤0.05% level.

Figure 1.  β-1,3-glucanase activity in control and pathogen (Alternaria brassicicola) inoculated 10 days (A) and one month (B) old arugula (Eruca sativa) plants viz. T-27 and RTM-2002. – • – T-27 control, – ○ – T-27 inoculated, –▾– RTM-2002 control, – ▿ – RTM-2002 inoculated. Each value represents the mean of four replicates with SE determined. Mean differences were significant at ≤0.05% level.

Figure 2.  Chitinase activity in control and pathogen (Alternaria brassicicola) inoculated 10 days (A) and one month (B) old arugula (Eruca sativa) plants viz. T-27 and RTM-2002. – • – T-27 control, – ○ – T-27 inoculated, – ▾ – RTM-2002 control, – ▿ – RTM-2002 inoculated. Each value represents the mean of four replicates with SE determined. Mean differences were significant at ≤0.05% level.

Figure 2.  Chitinase activity in control and pathogen (Alternaria brassicicola) inoculated 10 days (A) and one month (B) old arugula (Eruca sativa) plants viz. T-27 and RTM-2002. – • – T-27 control, – ○ – T-27 inoculated, – ▾ – RTM-2002 control, – ▿ – RTM-2002 inoculated. Each value represents the mean of four replicates with SE determined. Mean differences were significant at ≤0.05% level.

Figure 3.  Western blot of PR proteins showing the detection of β-1,3-glucanase activity from Eruca sativa after pathogen (A. brassicicola) infection in var. RTM-2002 (A) and T-27 (B) after different time intervals; lane 1: Marker, lane 2: 0 h control, lane 3: 4 h control, lane 4: 4 h inoculated, lane 5: 48 h control, lane 6: 48 h inoculated, lane 7: 168 h control, lane 8: 168 h inoculated.

Figure 3.  Western blot of PR proteins showing the detection of β-1,3-glucanase activity from Eruca sativa after pathogen (A. brassicicola) infection in var. RTM-2002 (A) and T-27 (B) after different time intervals; lane 1: Marker, lane 2: 0 h control, lane 3: 4 h control, lane 4: 4 h inoculated, lane 5: 48 h control, lane 6: 48 h inoculated, lane 7: 168 h control, lane 8: 168 h inoculated.

Figure 4.  Western blot of PR proteins showing the detection of chitinase activity from Eruca sativa after pathogen (A. brassicicola) infection in var. RTM-2002 (A) and T-27 (B) after different time intervals; lane 1: Marker, lane 2: 0 h control, lane 3: 4 h control, lane 4: 4 h inoculated, lane 5: 48 h control, lane 6: 48 h inoculated, lane 7: 168 h control, lane 8: 168 h inoculated.

Figure 4.  Western blot of PR proteins showing the detection of chitinase activity from Eruca sativa after pathogen (A. brassicicola) infection in var. RTM-2002 (A) and T-27 (B) after different time intervals; lane 1: Marker, lane 2: 0 h control, lane 3: 4 h control, lane 4: 4 h inoculated, lane 5: 48 h control, lane 6: 48 h inoculated, lane 7: 168 h control, lane 8: 168 h inoculated.

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