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Plant-Microorganism Interactions

Elicitation of tobacco alkaloid biosynthesis by disrupted spores and filtrate of germinating spores of the arbuscular mycorrhizal fungi Glomus etunicatum

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Pages 162-169 | Received 09 May 2012, Accepted 08 Jun 2012, Published online: 05 Jul 2012

Figures & data

Figure 1.  Nicotine, nornicotine, and anabasine concentrations in leaves and roots of Nicotiana tabacum plants after 3 or 10 days of elicitor solution applications (Control – plants without elicitor solution application; GS – elicitor solution from germinating spore exudates; and DS – elicitor solution from disrupted AMF spores). Different lower case letters denote statistically significant differences among treatments in leaves or roots, and uppercase letters are used to compare total (leaves + roots) alkaloid contents by the Tukey's test (5%). Error bars show the standard deviation (n=4).

Figure 1.  Nicotine, nornicotine, and anabasine concentrations in leaves and roots of Nicotiana tabacum plants after 3 or 10 days of elicitor solution applications (Control – plants without elicitor solution application; GS – elicitor solution from germinating spore exudates; and DS – elicitor solution from disrupted AMF spores). Different lower case letters denote statistically significant differences among treatments in leaves or roots, and uppercase letters are used to compare total (leaves + roots) alkaloid contents by the Tukey's test (5%). Error bars show the standard deviation (n=4).

Figure 2.  Relative transcript levels of tobacco alkaloid related-enzymes in relation to elongation factor EF-1α transcript levels in roots of Nicotiana tabacum plants after 3 or 10 days of elicitor solution applications (Control – plants without elicitor solution application; GS – elicitor solution from germinating spore exudates; and DS – elicitor solution from disrupted AMF spores). Significant differences among treatments at P<0.05 were determined by one-way analysis of variance (ANOVA) followed by the Tukey's test and are indicated by different letters. Error bars show the standard deviation (n=4).

Figure 2.  Relative transcript levels of tobacco alkaloid related-enzymes in relation to elongation factor EF-1α transcript levels in roots of Nicotiana tabacum plants after 3 or 10 days of elicitor solution applications (Control – plants without elicitor solution application; GS – elicitor solution from germinating spore exudates; and DS – elicitor solution from disrupted AMF spores). Significant differences among treatments at P<0.05 were determined by one-way analysis of variance (ANOVA) followed by the Tukey's test and are indicated by different letters. Error bars show the standard deviation (n=4).

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