Alteration in the gene expression of Glehnia littoralis seedlings exposed to culture filtrate of Penicillium citrinum KACC43900

Figures & data

Figure 1. Forty days old seedlings of G. littoralis treated with culture filtrate of fungal endophyte P. citrinum KACC43900 and (b) control treatment.

Figure 2. Comparison of 2DE profiles of crude protein extracts from fungal CF treated (a) and control (b) seedlings of G. littoralis. Image analysis showed difference in the intensity of 100 protein spots with mass range between 10 and 113 kDa. Fifty six spots with statistically significant difference were analyzed with MALDI-TOF MS for protein identification.

Table 1. Highly expressed proteins identified from G. littoralis seedlings treated with culture filtrate of P. citrinum.

Spot no.^a	Mol.wt./pI^b	Identification	Category	Plant^c	Accession no.^d	Fold^e
103	32.31/4.27	Chlorophyll a b binding protein	PAP	Apium graveolens	P92919	2.70
205	39.20/4.28	Ribosomal protein S3	PSAP	Halimeda simulans	AAV83771	1.94
1812	99.42/4.61	Hypothetical protein	UF	–	–	3.82
2305	45.55/4.86	Glyceraldehyde-3-phosphate dehydrogenase A subunit	GAP	Arabidopsis thaliana	NP_566796	5.35
2407	50.14/4.87	Phosphoglycerate kinase	GAP	Arabidopsis thaliana	NP_176015	3.97
2505	54.24/4.74	Ribulose-1,5-bisphosphate carboxylase/oxygenase	PAP	Arabidopsis thaliana	NP_051067	–
2706	81.85/4.71	Sex determination transformer protein 2 precursor	MP	Arabidopsis thaliana	AAD29784	1.89
2804	84.79/4.73	Ornithine decarboxylase	GAP	Arabidopsis thaliana	NP_001234616	2.76
3306	45.46/5.06	Glyceraldehyde-3-phosphate dehydrogenase A subunit	GAP	Arabidopsis thaliana	NP_566796	2.55
3402	49.60/4.94	Maturase K	PSAP	Medicago trancatula	XP_003621693	1.83
3505	51.53/4.95	Heat shock protein binding protein	MP	Arabidopsis thaliana	NP_849392	2.61
3711	71.75/5.06	ATP-synthase CF1 alpha subunit	EAP	Arabidopsis thaliana	NP_051044	3.03
3808	94.11/5.06	SKP1/ASK-interacting protein 16	GAP	Arabidopsis lyrata	XP_002892320	3.11
4004	20.51/5.19	Ribulose-1,5-bisphosphate carboxylase/oxygenase	PAP	Arabidopsis thaliana	NP_051067	3.13
4008	22.83/5.23	ATKT1 putative K ion transporter	TAP	Arabidopsis thaliana	AAF14830	2.14
4404	46.18/5.19	GBF2, G-box binding factor 2	PSAP	Arabidopsis thaliana	NP_192021	4.33
4502	53.40/5.11	Putative retroelement polyprotein	MP	Arabidopsis thaliana	AAG10817	2.01
4601	62.50/5.11	Leucine rich repeat family protein	GAP	Arabidopsis thaliana	NP_190892	2.79
4701	72.82/5.12	ATP-synthase CF1 alpha subunit	EAP	Arabidopsis thaliana	NP_051044	2.95
4801	105.76/5.10	ATP dependent CLPC heat shock protein	MP	Arabidopsis thaliana	NP_568746	2.05
4815	109.45/5.22	Phytochrome defective E	EAP	Arabidopsis thaliana	NP_193547	2.68
4816	113.64/5.23	NADPH-quinone oxidase-reductase subunit	EAP	Malva viscus arboreus	Q9MVL6	7.25
5002	10.37/5.36	UDP-glucose: glucosyltransferase	GAP	Arabidopsis thaliana	BAA34687	37.26
5107	30.08/5.42	Polypeptide of photosystem II	PAP	Oryza sativa	AAC98778	2.83
5109	27.58/5.46	Ribulose-1,5-bisphosphate carboxylase/oxygenase	PAP	Arabidopsis thaliana	NP_051067	1.68
5303	41.93/5.39	Ribulose-1,5-bisphosphate carboxylase activase	PAP	Nicotiana tabacum	S25484	2.68
5408	46.23/5.42	Putative polynucleo adenylyltransferase	PSAP	Arabidopsis thaliana	NP_197758	2.54
5409	46.57/5.47	Putative polynucleo adenylyltransferase	PSAP	Arabidopsis thaliana	NP_197758	3.65
5508	51.36/5.33	Glutamate-1-semialdehyde-2, 1-aminomutase	GAP	Arabidopsis thaliana	NP_201162	1.93
5605	64.75/5.38	WRKY34 microspore specific promoter/transcription factor	MP	Arabidopsis thaliana	NP_194374	2.06
5608	62.78/5.44	La domain containing protein	MP	Arabidopsis thaliana	NP_201411	3.08
5705	69.77/5.30	Acyl CoA thioesterase family protein		Arabidopsis thaliana	AEE81893	3.99
6103	28.79/5.59	K+ channel protein	TAP	Arabidopsis thaliana	AAA87294	1.81
6513	50.94/5.80	Glyceraldehyde-3-phosphate dehydrogenase B subunit	GAP	Arabidopsis thaliana	NP_174996	1.76
7212	38.37/6.78	Ribulose-1,5-bisphosphate carboxylase/oxygenase	PAP	Arabidopsis thaliana	NP_051067	1.98
7407	46.40/6.40	Glyceraldehyde-3-phosphate dehydrogenase A subunit	GAP	Arabidopsis thaliana	NP_566796	1.62
7805	98.24/6.13	Cobalamine independent methionine synthase	PSAP	Arabidopsis thaliana	NP_197294	1.73
8102	31.30/6.89	Gibberellin 3-oxidase	GAP	Solanum tuberosum	ACN89835	20.01
8801	85.28/6.80	ATP dependent RNA helicase, putative	PSAP	Arabidopsis thaliana	AAG51496	1.49
8805	110.68/6.98	Cobalamine independent methionine synthase	PSAP	Arabidopsis thaliana	NP_197294	2.08
8806	95.24/6.99	Cytochrome P450 family protein	GAP	Arabidopsis thaliana	AEE77974	2.73

Note: The proteins were identified by submitting MALDI-TOF MS data to the search database ProFound.

GAP, growth associated proteins; PSAP, protein synthesis and associated proteins; PAP, photosynthesis associated proteins; EAP, energy metabolism associated proteins; TAP, transport associated proteins; MP, miscellaneous proteins; UFP, proteins with unknown function; ATP, adenosine triphosphate.

^a Spot number on the gel image.

^b The actual mass and pI of the protein determined on the gel.

^c The plant of the highly matching proteins in the NCBI Protein database.

^d The accession numbers of the highly matching proteins in the NCBI Protein database.

^e Ratio of spot intensity on the gel of fungal CF treated samples and control samples.

Table 2. Uniquely expressed proteins identified from G. littoralis seedlings treated with culture filtrate of P. citrinum.

Spot no.^a	Mol.wt./pI^b	Identification	Category	Plant^c	Accession no.^d	Fold^e
1202	32.94/4.34	Unknown protein	UF	Arabidopsis thaliana	NP_177271	8.48
2302	44.58/4.74	Hypothetical protein	UF	Oryza sativa	EEE67072	–
3506	57.02/4.96	Toprim domain containing protein	GAP	Arabidopsis thaliana	AEE31259	2.61
3801	108.04/4.88	Polyprotein	MP	Oryza sativa	AAT85010	–
5003	22.56/5.36	Potassium transporter, putative	TAP	Arabidopsis thaliana	AAF14830	–
5007	22.62/5.49	Hypothetical protein	UF	–	–	–
5407	50.26/5.40	VCS nucleotide binding protein	PSAP	Arabidopsis thaliana	AEE75331	–
5511	57.14/5.38	Glycine rich protein	GAP	Arabidopsis thaliana	CAA77883	–
5512	58.28/5.40	Endonuclease/exonuclease/phosphatase family protein	PSAP	Arabidopsis thaliana	NP_181918	–
5514	52.77/5.46	WRKY34 microspore specific promoter/transcription factor	MP	Arabidopsis thaliana	NP_194374	–
5713	73.49/5.40	Ent-kaur 16-ene synthase	GAP	Arabidopsis thaliana	AEE36246	–
6806	98.25/5.85	Cobalamine independent methionine synthase	PSAP	Arabidopsis thaliana	NP_197294	–
7504	51.23/6.77	Unnamed protein	UF	–	–	–
7808	103.23/6.66	Beta-expansin EXPB4	GAP	Oryza sativa	AAK55466	–
8502	54.01/7.22	Citrate synthase like protein	GAP	Arabidopsis thaliana	NP_850415	–

Note: The proteins were identified by submitting MALDI-TOF MS data to the search database ProFound.

GAP, growth associated proteins; PSAP, protein synthesis and associated proteins; PAP, photosynthesis associated proteins; EAP, energy metabolism associated proteins; TAP, transport-associated proteins; MP, miscellaneous proteins; UFP, proteins with unknown function.

^a Spot number on the gel image.

^b The actual mass and pI of the protein determined on the gel.

^c The plant of the highly matching proteins in the NCBI Protein database.

^d The accession numbers of the highly matching proteins in the NCBI Protein database.

^e Ratio of spot intensity on the gel of fungal CF treated samples and control samples.

Figure 3. Proportion (%) of highly or uniquely expressed proteins groups (based upon their functions in plants) in fungal CF treated seedlings of G. littoralis. GAP, growth associated proteins; PSAP, protein synthesis and associated proteins; PAP, photosynthesis associated proteins; EAP, energy metabolism associated proteins; TAP, transport associated proteins; MP, miscellaneous proteins; UFP, proteins with unknown function.