GABA metabolism and ROS induction in lentil (Lens culinaris Medik) plants by synthetic 1,2,3-Thiadiazole compounds

Figures & data

Figure 1. Chemical structures of the three synthetic 1,2,3-thiadiazole compounds that were used in this study.

Table 1. Lentil shoot fresh weight (gm) in response to the three 1,2,3-thiadiazole compounds treatment.

	Compound I		Compound II		Compound III
Thiadiazole concentration	Jordan 1	Jordan 2	Jordan 1	Jordan 2	Jordan 1	Jordan 2
0 µg/L	2.6	2.1	2.45	2.06	2.2	1.94
5 µg/L	2.04	1.74	2.4	1.96	1.7	1.63
25 µg/L	1.92	1.45	1.8	1.65	1.54	1.25
50 µg/L	1.4	1.1	1.52	1.35	0.6	0.45
p-Value	.004	.001	.003	.002	.02	.026

Table 2. Lentil shoot dry mass (gm) in response to the three 1,2,3-thiadiazole compounds treatment.

	Compound I		Compound II		Compound III
Thiadiazole concentration	Jordan 1	Jordan 2	Jordan 1	Jordan 2	Jordan 1	Jordan 2
0 µg/L	1.9	1.73	1.85	1.74	1.76	1.62
5 µg/L	1.64	1.55	1.62	1.46	1.37	1.32
25 µg/L	1.53	1.32	1.44	1.33	1.06	1.01
50 µg/L	1.21	0.75	1.23	0.94	0.3	0.23
p-Value	.002	.008	.001	.004	.036	.04

Figure 2. The levels of malondialdehyde (MDA) in 10 days old seedlings of Jordan 1 and Jordan 2 lentil cultivars exposed to 1,2,3-thiadiazole compounds (I, II and III) independently determined by TBARS assay as described in materials and methods. MDA level was determined as nmol/mg fresh weight. (a) MDA levels in response to 1,2,3-thiadiazole compound I. (b) MDA levels in response to 1,2,3-thiadiazole compound II. (c) MDA levels in response to 1,2,3-thiadiazole compound III.

Figure 3. Gamma-aminobutyric acid (GABA) levels in Jordan 1 and Jordan 2 lentil cultivars sprayed with different concentrations of 1,2,3-thiadiazole compounds (I, II, and III) independently. GABA was extracted and determined according to the materials and methods. The level of GABA was determined as nmol/mg FW. (a) GABA levels in response to 1,2,3-thiadiazole compound I. (b) GABA levels in response to 1,2,3-thiadiazole compound II. (c) GABA levels in response to 1,2,3-thiadiazole compound III.

Figure 4. Total Carbohydrates levels in Jordan 1 and Jordan 2 lentil cultivars sprayed with different concentrations of 1,2,3-thiadiazole compounds (I, II,and III) independently. Carbohydrate levels were determined by using Anthron method as described in the materials and methods. The level of carbohydrate was determined as (mg/L)/FW. (a) Carbohydrates levels in response to1,2,3-thiadiazole compound I. (b) Carbohydrates levels in response to 1,2,3-thiadiazole compound II. (c) Carbohydrates levels in response to 1,2,3-thiadiazole compound III.

Figure 5. Total Protein levels in Jordan 1 and Jordan 2 lentil cultivars sprayed with different concentrations of 1,2,3-thiadiazole compounds (I, II,and III) independently. Protein levels were determined by using SMART BCA Protein Assay Kit according to the materials and methods. The level of Protein was determined as (μg/L)/FW. A) Proteins levels in response to 1,2,3-thiadiazole compound I. B) Proteins levels in response to 1,2,3-thiadiazole compound II. C) Proteins levels in response to 1,2,3-thiadiazole compound III.