Figures & data
Figure 1. Picture of Br, Be and Be (ABA) three weeks post-ABA treatment (11 weeks post flowering).
Figure 2. Physiological parameters of Br, Be and Be (ABA) three weeks post-ABA treatment (11 weeks post flowering). TA, titratable acid; TSS, total soluble solids. All the data were analyzed by SPSS, p < .05. Note: Due to the value of anthocyanin content was quite low, the data of anthocyanin content here were magnified 10 times.
Figure 3. The total intensity of all substances in the berries peel of Br, Be and Be (ABA) three weeks post-ABA treatment (11 weeks post flowering). This chart was made by MSconvertGUI software.
Figure 4. The chart to show how delphinidin-3-O-monoglucoside was found. A: a mass spectrometry detected time peak intensity. B: relative molecular weight peak intensity and its hydrogenation peaks intensity. C: two stage mass spectrometry peak intensity.
Figure 5. The type of anthocyanins which were detected by Triple TOF 5600+ AB, the peak height shows similar trend with the area (R2=0.9982). Note: Cya-3-O-mon, cyanidin-3-O-monoglucoside; Peo-3-O-mon, peonidin-3-O-monoglucoside; Cya-3,5-O-dig, cyanidin-3,5-O-diglucoside; Peo-3-O-cou, peonidin-3-O-coumarylglucoside; Mal-3-O-mon, malvidin-3-O-monoglucoside; Del-3-O-mon, delphinidin-3-O-monoglucoside; Peo-3-O-ace, peonidin-3-O-acetylglucoside; Pet-3,5-O-dig, petunidin-3,5-O-diglucoside; Mal-3-O-cou, malvidin-3-O-coumarylglucoside; Mal-3-O-ace, malvidin-3-O-acetylglucoside.
Table 1. The type of anthocyanins and areas which were detected by Triple TOF 5600+ AB
Figure 6. RT-qPCR of anthocyanin biosynthetic pathway related genes. The Y-axis represents gene relative expression and the X-axis represents weeks post flowering.
Figure 7. The probable anthocyanin biosynthesis mechanism of deeper coloring bud mutation in (Br) and Be (ABA) cultivars. The whole process takes place in natural conditions; the red pathway may demonstrate the effects of ABA. Del, delphinidin; Pet, petunidin; Mal, malvidin; Cya, cyanidin; Peo, peonidin.
