OsRuvB transgene induces salt tolerance in pigeon pea

Figures & data

Figure 1. 1.5% Agarose gel showing PCR amplification of OsRuvB gene using gene specific primers (Lanes: M: 1 kb DNA ladder (Fermentas); −ve: negative control; 1–70: T₀ plant samples; +ve: positive control). Full length gels are displayed in supplementary figure 1. Different gels separated by white spacing in between.

Figure 2. Southern blot analysis of transgenic plants (Lanes: –ve: DNA from Wild type plant; +ve: DNA from pCAMBIA1301-OsRuvB; 1–6: DNA from transgenic lines where 1: L-66, 2: L-37, 3: L-32, 4: L-17, 5: L-107, 6: L-10). Full length blots are displayed in supplementary figure 2. Different blots separated by white spacing in between.

Table 1. Seed yield (g/plant) in T₀ transgenic and wild type pigeon pea plants.

S. No	Plant no.	Seed yield (g/plant)
1	L-10	5.01
2	L-17	6.78
3	L-20	3.13
4	L-32	6.13
5	L-37	8.41
6	L-65	2.50
7	L-66	6.43
8	L-67	3.16
9	L-69	3.79
10	L-77	3.68
11	L-85	3.54
12	L-87	3.65
13	L-88	2.25
14	L-93	3.80
15	L-96	3.19
16	L-98	3.74
17	L-102	3.16
18	L-104	4.03
19	L-107	5.70
20	L-108	3.62
21	L-109	2.68
22	L-113	4.64
23	L-114	3.26
24	L-116	2.82
25	L-118	3.23
26	Wild type	3.62

Note: C.D. at 5% 0.001.

Table 2. Segregation analyses of the transgene in T₁ progeny of transgenic pigeon pea based on PCR analysis.

S. No.	Line	No. of T1 plants screened	OsRuvB +ve	OsRuvB −ve	Observed ratio	χ^2-value	p-value
1	L-10	43	31	12	2.6:1	0.21	0.64
2	L-17	42	32	10	3.2:1	0.12	0.72
3	L-32	47	34	13	2.6:1	0.10	0.75
4	L-37	50	36	14	2.6:1	0.09	0.76
5	L-66	45	33	12	2.8:1	0.11	0.74
6	L-107	36	33	3	11:1	0.52	0.47

Figure 3. Wild type and T₁ transgenic pigeon pea plants 8 days after giving 75 mM salt treatment.

Figure 4. Effect of salt stress (75 mM NaCl) on (a) Relative Water content (%), (b) Membrane Injury Index (%), (c) MDA content (µmol/g FW), (d) Chlorophyll content (mg/g FW), (e) Proline content (µmol/g FW), (f) Total Soluble Sugar content (mg/g FW), (g) Catalase activity (mM/min/g FW), (h) Peroxidase activity (mM/min/g FW) in wild type and T₁ transgenic pigeon pea lines. (Detailed analysis given in supplementary table 1–8).