Preliminary evaluates of silica/β-TCP/PLGA microspheres for dentin regeneration in vivo

ABSTRACT

Silica/β-tri-calcium phosphate/poly (lactic-co-glycolic acid) microspheres, a type of bioactive and biodegradable materials, were produced by a double emulsion and solvent evaporation methods. The biological activity of the microspheres to promote dentin regeneration was investigated in vivo. First, the ionic concentration of silicon in the microspheres was detected by inductively coupled plasma optical emission spectrometry. The results showed the release of silicon continuously and without any obvious burst. Second, the microspheres with or without human dental pulp cells adhered were transplanted into subcutaneous pockets of nude mice to evaluate the bioactivity. The implanted material specimens were obtained after 6 weeks. The specimens were evaluated after H&E staining and toluidine blue staining. The results presented the cells ingrowth of the scaffold material and collagenous tissues around the microspheres. It was demonstrated that the material had outstanding biocompatibility and bioactivity.

KEYWORDS:

• Silica
• microspheres scaffold
• biological activity

Acknowledgements

This study was supported by grants from the Natural Science Foundation of China (Project nos. 51772007).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This study was supported by grants from the Natural Science Foundation of China [Project No. 51772007].