Green synthesis of silver nanoparticles from purple acid phosphatase apoenzyme isolated from a new source Limonia acidissima

Figures & data

Figure 1. Formation of silver nanoparticles after sonication.

Figure 2. TEM images of silver/silver oxide nanoparticles. Avg particle size (25–45 nm).

Figure 3. UV–Visible spectra of silver/silver oxide nanoparticles. All the experiments were performed at room temperature (∼25 °C).

Figure 4. FTIR spectra of silver/silver oxide nanoparticles.

Figure 5. XRD spectra of silver/silver oxide sample.

Table 1. Summary of purification of PAP from 1 kg tubers namely Limonia acidissima.

Steps	Protein (mg)	Activity units (µm/ml/min)	Specific activity (units/mg)	Yield
Crude Juice	78,809	144,438	1.4	100
0% to 45% ammonium sulphate pptn.	2156	117,516	38.1	80
85% ammonium sulphate pptn	2156	117,516	38.1	80
Acid treatment (pH 4.0)	1400	111,118	55.5	76
Acid treatment (pH 6.0)	959	106,362	77.7	72
Gel filtration Sephadex G-100	62	24,603	276	22
Affinity chrom. Con A	56	28,672	358	20
Gel filtration Sephadex G-100	50	28,672	402	20

Table 2. Antimicrobial activity of silver nanoparticles from enzyme against test organisms.

		Zone of inhibition (mm)
Organism	Gram character	Control	PAP	AgNPs
Escherichia coli	Gram negative	–	∼1.1	7
Staphylococcus aureus	Gram positive	–	∼1.5	9
Pseudomonas aeruginosa	Gram negative	–	∼1.3	11