Mining of lactic acid bacteria from traditional yogurt (Mast) of Iran for possible industrial probiotic use

Figures & data

Figure 1. The detailed overlay of the region-wise number of identified strains.

Figure 2. Phylogenetic tree of the four native lactic acid bacteria strains found in Mast based on rRNA sequence.

Table 1. The biochemical and morphological characteristics of the isolates.

Strain	Glucose	Mannose	Fructose	Trehalose	Lactose	Arabinose	Sucrose	Xylorose	Galactose	Heat reaction	Motility	Catalase	Oxidase	Growth temp, °C
														45	15
L1	+	+	+	+	+	−	+	−	+	+	−	−	−	−	+
L2	+	+	+	−	+	−	−	−	−	+	−	−	−	+	−
L3	+	+	+	+	−	−	+	−	−	+	−	−	−	+	−
L4	+	+	+	+	+	−	+	−	+	+	−	−	−	−	+
L5	+	+	+	+	−	−	+	−	−	+	−	−	−	+	−
L6	+	+	+	+	+	−	+	−	+	+	−	−	−	+	−
L7	+	+	+	+	+	−	+	−	+	+	−	−	−	−	+
L8	+	+	+	−	+	−	−	−	−	+	−	−	−	+	−

+/− refers to Degree Celsius.

Table 2. Bile salt resistant activity of Isolated LAB and reference Lactobacillus bulgaricus.

Strain	Strain growth in bile salt (%)
	0.3, %	0.5, %	1.0, %
L1	3.23 ± 0.22	1.94 ± 0.15	1.34 ± 0.28
L2	6.56 ± 0.15	5.23 ± 0.25	3.78 ± 0.54
L3	3.23 ± 0.12	2.31 ± 0.11	1.01 ± 0.15
L4	15.65 ± 2.26	13.01 ± 1.77	7.22 + 0.52
L5	9.32 ± 1.23	6.34 ± 0.65	4.22 ± 1.11
L6	18.92 ± 2.32	13.56 ± 3.23	10.22 ± 1.31
L7	2.92 ± 0.17	1.95 ± 0.11	1.07 ± 0.15
L8	6.54 ± 0.33	4.34 ± 0.24	2.54 ± 0.15
Lactobacillus bulgaricus	4.23  ± 0.15	2.65  ± 0.22	1.94  ± 0.42