AMP-activated protein kinase mediates glycolysis in post-mortem breast muscle of broilers

Figures & data

Figure 1. Effects of activation of AMPK by AICAR or Compound C on AMPK phosphorylation (p-AMPK) level in post-mortem breast muscle of broiler. Results are presented as mean ± standard error (n = 6). ^a,b,cValues in the same row with different letters are significant different (p < 0.05). CON, control group, broilers injected with same volume of vehicle solution phosphate buffer saline; AICAR group, broilers were intraperitoneally injected with AICAR (5 mg/kg body weight); Compound C group, broilers were intraperitoneally injected with Compound C (1 mg/kg body weight).

AMPK: AMP-activated protein kinase; AICAR: 5-aminoimidazole-4-carboxamide ribonucleoside; CON: Control.

Table 1. Effects of activation of AMPK by AICAR or Compound C on meat quality in broiler breast muscle.

Item^A	CON	AICAR	Compound C
pH45min	6.34 ± 0.00^a	6.16 ± 0.09^b	6.56 ± 0.08^c
pH24h	5.78 ± 0.10^a	5.24 ± 0.04^b	5.91 ± 0.05^a
L*	51.84 ± 2.29^a	55.44 ± 0.57^b	49.06 ± 1.46^c
a*	10.43 ± 0.45^a	10.33 ± 0.37^a	10.29 ± 0.28^a
b*	8.88 ± 0.21^a	8.82 ± 0.60^a	8.90 ± 0.27^a
Drip loss, %	6.35 ± 0.80^a	8.18 ± 0.48^b	6.15 ± 0.88^a

^AResults are presented as mean ± standard error (n = 6).

^a,b,cValues in the same row with different letters are significant different (p < 0.05). CON, control group, broilers injected with same volume of vehicle solution phosphate buffer saline; AICAR group, broilers were intraperitoneally injected with AICAR (5 mg/kg body weight); Compound C group, broilers were intraperitoneally injected with Compound C (1 mg/kg body weight); L*, lightness; a*, redness; b*, yellowness.

AMPK: AMP-activated protein kinase; AICAR: 5-aminoimidazole-4-carboxamide ribonucleoside.

Figure 2. (A–E) Effects of activation of AMPK by AICAR or Compound C on glycolytic metabolites in post-mortem breast muscle of broiler. Results are presented as mean ± standard error (n = 6). ^a,b,cValues in the same row with different letters are significant different (p < 0.05). CON, control group, broilers injected with same volume of vehicle solution phosphate buffer saline; AICAR group, broilers were intraperitoneally injected with AICAR (5 mg/kg body weight); Compound C group, broilers were intraperitoneally injected with Compound C (1 mg/kg body weight); G-6-P, Glucose-6-Phosphate.

AMPK: AMP-activated protein kinase; AICAR: 5-aminoimidazole-4-carboxamide ribonucleoside; CON: Control.

Figure 3. (A–D) Effects of activation of AMPK by AICAR or Compound C on glycolytic enzymes activity in post-mortem breast muscle of broiler. Results are presented as mean ± standard error (n = 6). ^a,b,cValues in the same row with different letters are significant different (p < 0.05). CON, control group, broilers injected with same volume of vehicle solution phosphate buffer saline; AICAR group, broilers were intraperitoneally injected with AICAR (5 mg/kg body weight); Compound C group, broilers were intraperitoneally injected with Compound C (1 mg/kg body weight); HK, hexokinase; PFK-1, phosphofructokinase-1; PK, pyruvate kinase; LDH, lactate dehydrogenase.

AMPK: AMP-activated protein kinase; AICAR: 5-aminoimidazole-4-carboxamide ribonucleoside; CON: Control.

Data availability statement

The original data of the paper are available upon request from the corresponding author.