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Production Physiology and Biology

Lactoferrin concentration in bovine milk: validation of radial immunodiffusion technique, sources of variation, and association to udder health status

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Pages 230-238 | Received 19 Dec 2022, Accepted 09 Feb 2023, Published online: 28 Feb 2023

Figures & data

Table 1. Repeatability relative standard deviation (RSDr, %; n = 15), reproducibility relative standard deviation (RSDR, %; n = 45) and z-scores (z) for lactoferrin concentration (mg/dL) in raw milk (RM), raw frozen milk (RFM) and raw milk with preservative added (RPM).

Table 2. Descriptive statistics of milk lactoferrin concentration, production-related traits and milk quality traits in Holstein Friesian and Simmental cows.

Table 3. Pearson correlations of milk lactoferrin with milk yield and quality traits.

Table 4. F-values and significance of fixed effects, and variance accounted by herd test-day nested within breed effect (σ2HTD(Breed), %) for lactoferrin concentration, milk yield and quality traits.

Figure 1. Least squares means (with standard error) of milk lactoferrin concentration across classes of lactation stage (A) and parity classes (B). Means with different letters differ significantly (p <.05).

Figure 1. Least squares means (with standard error) of milk lactoferrin concentration across classes of lactation stage (A) and parity classes (B). Means with different letters differ significantly (p <.05).

Figure 2. Distribution of the data and least squares means of milk lactoferrin concentration (mg/dL) according to udder health status classification proposed by Zecconi et al. (Citation2019): healthy (SCC ≤ 200 cells/µL and DSCC ≤ 66.3%), susceptible (SCC ≤ 200 cells/µL and DSCC ≥ 66.3%), mastitic (SCC ≥ 200 cells/µL and DSCC ≥ 66.3%) and chronic (SCC ≥ 200 cells/µL and DSCC ≤ 66.3%). Means with different letters differ significantly (p <.05).

Figure 2. Distribution of the data and least squares means of milk lactoferrin concentration (mg/dL) according to udder health status classification proposed by Zecconi et al. (Citation2019): healthy (SCC ≤ 200 cells/µL and DSCC ≤ 66.3%), susceptible (SCC ≤ 200 cells/µL and DSCC ≥ 66.3%), mastitic (SCC ≥ 200 cells/µL and DSCC ≥ 66.3%) and chronic (SCC ≥ 200 cells/µL and DSCC ≤ 66.3%). Means with different letters differ significantly (p <.05).

Data availability statement

None of the data were deposited in an official repository. The data that support the findings presented in this study are available from the first author or corresponding author upon reasonable request.