Figures & data
Figure 1. N-glycan profiles, %Galactosylated, %Afucosylated and %High Mannosylated, and %Charged levels of Remicade®, Flixabi®, and Remsima®. (A) N-glycan profiles of Remicade®, Flixabi®, and Remsima®. EU, emission unit. (B) Percentage of galactosylated glycans. (C) Percentage of afucose and high mannosylated glycans. (D) Percentage of charged glycans. Light color dots: Individual data; Dark color dots: Mean data with mean value and standard deviation; Bar: 95% confidence interval of mean.
![Figure 1. N-glycan profiles, %Galactosylated, %Afucosylated and %High Mannosylated, and %Charged levels of Remicade®, Flixabi®, and Remsima®. (A) N-glycan profiles of Remicade®, Flixabi®, and Remsima®. EU, emission unit. (B) Percentage of galactosylated glycans. (C) Percentage of afucose and high mannosylated glycans. (D) Percentage of charged glycans. Light color dots: Individual data; Dark color dots: Mean data with mean value and standard deviation; Bar: 95% confidence interval of mean.](/cms/asset/ba213d58-9abc-44ba-ae50-1d0c219795d2/kmab_a_1337620_f0001_oc.gif)
Figure 2. FcγRIIIa binding activity of Remicade®, Flixabi®, and Remsima®. (A) Percentage of relative binding activity using surface plasmon resonance. (B) Percentage of relative binding activity using AlphaScreen. (C) Percentage of relative binding activity using fluorescence-activated cell sorting. Light color dots: Individual data; Dark color dots: Mean data with mean value and standard deviation; Bar: 95% confidence interval of mean.
![Figure 2. FcγRIIIa binding activity of Remicade®, Flixabi®, and Remsima®. (A) Percentage of relative binding activity using surface plasmon resonance. (B) Percentage of relative binding activity using AlphaScreen. (C) Percentage of relative binding activity using fluorescence-activated cell sorting. Light color dots: Individual data; Dark color dots: Mean data with mean value and standard deviation; Bar: 95% confidence interval of mean.](/cms/asset/1c7303fc-75d5-43ec-a189-fa960dedff1b/kmab_a_1337620_f0002_oc.gif)
Figure 3. Antibody-dependent cell-mediated cytotoxicity of Remicade®, Flixabi®, and Remsima®. (A) CD16-positive cells (Count %) and mean fluorescence intensities (MFI) in CD3 negative populations from healthy donor peripheral blood mononuclear cells and NK92-CD16a cell line. APC, Allophycocyanin; APC-A, Area of APC detection. (B) Percentage of relative ADCC activity using a NK92-CD16a cell line. (C) Percentage of relative ADCC activity using PBMC from healthy human donors. Light color dots: Individual data; Dark color dots: Mean data with mean value and standard deviation; Bar: 95% confidence interval of mean.
![Figure 3. Antibody-dependent cell-mediated cytotoxicity of Remicade®, Flixabi®, and Remsima®. (A) CD16-positive cells (Count %) and mean fluorescence intensities (MFI) in CD3 negative populations from healthy donor peripheral blood mononuclear cells and NK92-CD16a cell line. APC, Allophycocyanin; APC-A, Area of APC detection. (B) Percentage of relative ADCC activity using a NK92-CD16a cell line. (C) Percentage of relative ADCC activity using PBMC from healthy human donors. Light color dots: Individual data; Dark color dots: Mean data with mean value and standard deviation; Bar: 95% confidence interval of mean.](/cms/asset/5850bb0a-7fc3-4eba-b094-c2e437d233e7/kmab_a_1337620_f0003_oc.gif)
Figure 4. Correlation between percentage afucose plus percentage high mannosylated (HM) glycans and Fc functions for Remicade®, Flixabi®, and Remsima®. (A) Correlation between %afucose plus %HM glycan and %relative FcγRIIIa binding activity. (B) Correlation between %afucose plus %HM glycan and %relative ADCC activity.
![Figure 4. Correlation between percentage afucose plus percentage high mannosylated (HM) glycans and Fc functions for Remicade®, Flixabi®, and Remsima®. (A) Correlation between %afucose plus %HM glycan and %relative FcγRIIIa binding activity. (B) Correlation between %afucose plus %HM glycan and %relative ADCC activity.](/cms/asset/989d82b4-3505-4a42-8732-6ed02f54b9b7/kmab_a_1337620_f0004_oc.gif)
Table 1. %Afucose plus high mannosylated glycan and %relative ADCC activity of infliximab products. %RBA: %relative biological activity.
Figure 5. Fab-related TNF-α binding and TNF-α neutralization activities of Remicade®, Flixabi®, and Remsima®. (A) Percentage of relative binding activity assessed by fluorescence resonance energy transfer. (B) Percentage of relative potency measured by a luciferase reporter gene assay. Light color dots: Individual data; Dark color dots: Mean data with mean value and standard deviation; Bar: 95% confidence interval of mean.
![Figure 5. Fab-related TNF-α binding and TNF-α neutralization activities of Remicade®, Flixabi®, and Remsima®. (A) Percentage of relative binding activity assessed by fluorescence resonance energy transfer. (B) Percentage of relative potency measured by a luciferase reporter gene assay. Light color dots: Individual data; Dark color dots: Mean data with mean value and standard deviation; Bar: 95% confidence interval of mean.](/cms/asset/f3f725f5-7939-4231-9426-17278df00f99/kmab_a_1337620_f0005_oc.gif)