Quantification of sixteen cannabinoids in hemp seed edible oils and the influence of thermal processing on cannabinoid contents and profiles

Figures & data

Table 1. Overview of the quality control parameters.

Analyte	Limit of quantification (LOQ)^a		Recovery in %^b	Precision in %
	ng mL^−1	mg kg^−1
CBC	0.5	0.025	91 ± 11	12
CBCA	0.5	0.025	106 ± 6	6
CBD	0.25	0.013	112 ± 3	3
CBDA	0.25	0.013	127 ± 4	3
CBDV	0.25	0.013	117 ± 2	2
CBDVA	0.5	0.025	120 ± 4	3
CBG	0.25	0.013	118 ± 9	8
CBGA	0.25	0.013	125 ± 2	2
CBL	0.1	0.003	90 ± 4	5
CBLA	0.5	0.025	122 ± 4	3
CBN	0.25	0.013	105 ± 3	3
CBNA	0.25	0.013	120 ± 9	8
Δ9-THC	0.1	0.003	96 ± 2	3
Δ8-THC	0.25	0.013	96 ± 3	3
THCA	0.5	0.025	117 ± 2	2
THCV	0.1	0.003	109 ± 2	2
THCVA	0.25	0.013	121 ± 4	3

^a The determination of the limit of quantification was carried out via the signal-to-noise ratio according to DIN32645. The conversion to mg kg⁻¹ has been made taking into account the dilution and solvation of 200 mg of oil sample to a total volume 10 mL with acetone.

^b To investigate the recovery, 200 mg of blank matrix sunflower seed oil were spiked with 200 µL cannabinoid standard mix (c = 1 µg mL⁻¹), corresponding to an absolute level of 200 ng. The spiked sample was dissolved and prepared as described in methods.

Figure 1. Cannabinoid contents in mg kg⁻¹ in the 30 investigated hemp seed oils. For each oil, the error bars represent the sum of the standard deviations for each individual cannabinoid from a duplicate workup, each measured at two dilution levels (n = 4). The individual values are listed in Table S3.

Figure 2. Percentage plot of cannabinoid profiles in each hemp seed oil sample.

Figure 3. Sum of and individual cannabinoid contents in mg kg⁻¹ in thermally processed hemp seed oils heated in vials that were a) non-capped and b) capped. The black dots represent the ratio of acidic and neutral cannabinoids (A/N) for the given samples (see secondary y-axis). For each sample, the error bars represent the sum of the standard deviations for each individual cannabinoid from a duplicate workup, each measured in two dilution levels (n = 4). The individual values are listed in Table S4 for a) and in Table S5 for b). ‘n. p.’ is short for ‘not processed’.