Biopreservation potential of Enterococcus faecalis isolated from Italian traditional raw milk cheeses

Figures & data

Table 1. Indicator organisms used in the antimicrobial activity assay.

Cepas de referencia utilizada para la identificacion de la actividad antimicrobiana.

	Source	Strain
Listeria monocytogenes	ATCC	9525
Staphylococcus aureus	ATCC	19095
Bacillus cereus	ATCC	14579
Escherichia coli	ATCC	8739
Clostridium sporogenes	ATCC	3584
Streptococcus thermophilus	CNR ISPA	BT63
Lactobacillus delbrueckii subsp. bulgaricus	DSMZ	20081
Lactobacillus fermentum	DSMZ	20052
Lactobacillus helveticus	DSMZ	20075
Lactobacillus rhamnosus	CNR ISPA	BT75
Lactobacillus reuteri	DSMZ	55730
Leuconostoc mesenteroides subsp. mesenteroides	DSMZ	20343
Lactococcus lactis subsp. cremoris	CNR ISPA	QL2
Pediococcus acidilactici	CNR ISPA	GP19
Enterococcus faecium	CNR ISPA	BT232
Enterococcus faecalis	CNR ISPA	192
Enterococcus faecalis	CNR ISPA	FK1
Enterococcus faecalis	CNR ISPA	Mora 13

Notes: ATCC, American Type Culture Collection; DSMZ, German Collection of Microorganisms and Cell Cultures; CNR ISPA, CNR ISPA strain collection.

Notas: ATCC, Colección Americana de Cultivos Tipo; DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen; CNR ISPA, Colecion CNR ISPA de Cultivos Tipo.

Figure 1. Dendrogram derived from profiles by the repetitive extragenic palindromic-PCR (rep-PCR) (a) and by the random amplification of polymorphic DNA (RAPD-PCR) generated with primers M13 and D11344 (b). The profile grouping was done with the BioNumeric 5.0 software package, using the unweighted pair group method with arithmetic averages (UPGMA) cluster analysis.

Dendrograma generado a partir de perfiles genéticos por PCR-palindrómico extragénico repetitivo (rep-PCR) (a) y por amplificación aleatoria de ADN polimórfico (RAPD-PCR) obtenidos con cebadores M13 y D11344 (b). Los perfiles fueron agrupados usando el software BioNumeric 5,0, usando el método de agrupación por media aritmética no ponderada (UPGMA).

Table 2. Prevalence of antimicrobial activity and enterocin genes in E. faecalis isolates in relation to their geographical and dairy origin.

Actividad antimicrobiana y genes para la producción de enterocinas de E. faecalis aisladas a partir de queso de leche cruda.

Strain	Geographical area	Dairy origin	Lm	Sa	Ec	Bc	Cs	St	Ldb	Lh	Lf	Lr	Lmm	Ef	Ef	Ent. genes
AA4	Brembana Valley	Goat milk cheese							+	+
AA8, AA11	Valtellina	Goat milk cheese				+			+	+
R29, R31, R169	Cuneo	Raschera cheese				+	+
R217, R219, R221	Cuneo	Raschera cheese				+
ID49	Cremona	Milk							+	+
ID136	Aosta Valley	Milk				+		+						+
ID143A	Aosta Valley	Milk				+		+
ID160	Aosta Valley	Milk				+
TO1	Brembana Valley	Formai de Mut cheese												+	+
TO6	Brembana Valley	Formai de Mut cheese							+	+
S13	Aosta Valley	Fontina cheese				+		+
VC12, VC17, VC 42	Valtellina	Valtellina Casera cheese	+		+				+	+			+			entAS48
VC49	Valtellina	Valtellina Casera cheese	+		+			+	+	+			+			entAS48
VC85	Valtellina	Valtellina Casera cheese			+		+	+	+	+	+	+		+
VS353	Seriana Valley	Formagèla Valseriana cheese					+		+	+
VS368	Seriana Valley	Formagèla Valseriana cheese					+
VS382B	Seriana Valley	Formagèla Valseriana cheese								+
VS485	Seriana Valley	Formagèla Valseriana cheese	+	+	+				+	+			+			entAS48
VS491	Seriana Valley	Formagèla Valseriana cheese							+	+
SE105, SE 120	Alto Lario	Semuda cheese					+		+	+
SE134, SE182, SE198, SE200, SE201	Alto Lario	Semuda cheese				+
SV6, SV38, SV56, SV83A	Scalve Valley	Formaggella Val di Scalve cheese							+	+
SV70A	Scalve Valley	Formaggella Val di Scalve cheese	+		+				+	+			+			entAS48

Notes: Lm, L. monocytogenes; Sa, S. aureus; Ec, E. coli; Bc, B. cereus; Cs, Cl. sporogenes; St, St. thermophilus; Ldb, Lb. delbrueckii subsp. bulgaricus; Lh, Lb. helveticus; Lf, Lb. fermentum; Lr, Lb. reuteri; Lmm, Ln. mesenteroides subsp. mesenteroides; Ef, E. faecalis; E. faecalis; Ent. genes, enterocin genes; +, positive. Strains in bold: strains owning activity against undesirable bacteria, but ineffective against LAB.