Simultaneous consumption of green and black tea infusions from Cnidoscolus aconitifolius leaves with metformin treatment improves the health outcome in type II diabetic rats

Figures & data

Figure 1. Total phenol content (TPT) of C. aconitifolius tea infusions.

GTC: Green tea, BTC: Black tea. a and b: Bands with different letters are significantly different at p < .05.

Figure 2. Total flavonoid (TFT) content of C. aconitifolius tea infusions.

GTC: Green tea, BTC: Black tea. a and b: Bands with different letters are significantly different at p < .05.

Figure 3. Evolution of the antiradical activity of C. aconitifolius tea infusions at different concentrations compared to vitamin C. GTC: Green tea, BTC: Black tea, Vit C: Vitamin C.

Figure 4. Evolution of the reducing power of C. aconitifolius tea infusions compared to BHT.

Figure 5. Changes in the hydroxyl radical inhibitory capacity of tea infusion at different concentrations compared to that of BHT.

Table 1. Changes in the FBG (g/L) during the treatment.

Groups	Day 0	Day 7	Day 14	Day 21	Day28	Difference
T-	0.81 ± 0.4^ɑ	0.91 ± 0.3^ɑ	0.86 ± 0.2^ɑ	0.83 ± 0.3^ɑ	0.90 ± 0.2^ɑ	0.09^a
T+	3.62 ± 0.4^c	3.88 ± 0.2^d	4.06 ± 0.4^e	4.86 ± 0.4^d	5.1 ± 0.7^d	1.48^b
Met	3.37 ± 0.5^b	3.09 ± 0.4^c	2.61 ± 0.6^d	2.10 ± 0.5^e	1.65 ± 0.32^c	−1.72^c
Met + GTC	3.68 ± 0.5^b	2.93 ± 0.5^b	2.31 ± 0.3^b	1.74 ± 0.4^b	1.29 ± 0.38^b	−2.39^d
Met + BTC	3.81 ± 0.7^b	3.01 ± 0.3^bc	2.43 ± 0.6^c	1.77 ± 0.5^b	1.33 ± 0.28^b	−2.48^d

T-: Healthy control; T+: Untreated diabetics; MET: Diabetics treated with metformin; Met +GTC: Diabetics treated with metformin and green tea; Met +BTC: Diabetics treated with metformin and black tea. Difference = Day 28 – Day 0. a, b and c: In the same column, values with different letters differ significantly (p < 0.05).

Figure 6. Serum concentration of triglycerides, total cholesterol, HDL cholesterol and LDL cholesterol in animals after 28 days of treatment. T (-): Healthy control; T (+): untreated diabetics; MET: Metformin; GTC: Green tea; BTC: Black tea. a, b, c and d: On the same graph, bands with different letters differ significantly (p < .05).

Figure 7. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total protein after 28 days of treatment. T (-): Healthy control; T (+): untreated diabetics; MET: Metformin; GTC: Green tea; BTC: Black tea. a, b and c: On the same graph, bands with different letters differ significantly (p < .05).

Figure 8. Serum urea and creatinine concentrations in animals after 28 days of treatment. T (-): Healthy control; T (+): untreated diabetics; MET: Metformin; GTC: Green tea; BTC: Black tea. a and b: On the same graph, bands with different letters differ significantly (p < .05).