Development of a colloidal gold immunochromatographic strip for rapid detection of ribavirin in pig urine

Figures & data

Figure 1. Chemical structure of ribavirin.

Figure 2. The route of synthetic synthesis the hapten for ribavirin.

Figure 3. Structure of strips for immune chromatography.

Figure 4. Illustration of typical test strip results.

Figure 5. SDS-PAGE of carrier proteins and conjugates(Lane 1: ri-BSA 5-fold dilution; Lane 2: ri-BSA 2-fold dilution; Lane 3: BSA 5-fold dilution; Lane 4: BSA 2-fold dilution).

Figure 6. Inhibition curve of Ri competitive and Ri competitive in pig urine samples.

Table 1. Cross-reactivity of other drugs/metabolites with the monoclonal antibody.

Compounds	IC50(ng/mL)	CR(%)
moroxydine hydrochloride	>100000	<0.1
acyclovir	>100000	<0.1
1,2,4-triazole-3-carbox amide	>100000	<0.1
1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxylic acid	>100000	<0.1

Table 2. The rate of recovery of the sample using ELISA.

			CVs(%)
Spiked level	Recovery(%)	SD(x = 10)	Intra-batch (x = 10) Inter-batch (x = 3)
0.1 ng/mL	99	0.029	4.49	3.15
1 ng/mL	95	0.009	1.92	3.16
10 ng/mL	77.3	0.010	3.17	3.14

Table 3. The relation of particle size and amount of sodium citrate added.

Chlorogold acid :Trisodium citrate	Color	Absorption peak of colloidal gold particles
1: 2	Light red	519
1:1.5	red	523
1:0.8	Wine red	528
1:0.6	Purple red	533

Figure 7. Images of the RBV analysis using the GCI strip assay in PBS (1 = 0, 2 = 1 ng/mL, 3 = 2 ng/mL, 4 = 3 ng/mL, 5 = 5 ng/mL).

Table 4. The repeatability of the colloidal gold strip.

Batch	Negative samples			Positive samples
	1	2	3	4	5	6
Batch one Batch two Batch three	– – –	– – –	– – –	+ + +	+ + +	+ + +

“−” was negative “+” was positive.