Figures & data
Figure 1. Effects of SHT on (A) cell growth, (B) RANKL-induced TRAP activity and (C) formation of TRAP-positive multinucleated osteoclasts in RAW264.7 cells. RAW264.7 cells were placed in a 96-well plate at a density of 1×103 cells/well with or without RANKL (100 ng/mL). The cells were incubated with different concentrations of SHT (25–200 µg/mL) for 3 days, and multinucleated osteoclasts were observed under a microscope. Cell viability was tested with CCK-8 assay. TRAP activity and staining were performed as described in Materials and methods. * P<0.05; ** P<0.01.
Table 1. Effect of SHT on RANKL included in RNA expression differentiation related genes in RAW264.7 cells
Figure 2. Change in body weight of rats during SHT administration for 12 weeks. The body weights of OVX and SHT (0.6 g/kg BID) groups were significantly increased for 12 weeks when compared to the sham group (P<0.01).
Table 2. Effect of SHT on RANKL uterine weight and blochemical parameters in serum of ovx rats
Figure 3. Histomorphological analysis of (A) femur (× 20) and (B) uterus (× 40). After SHT administration (0.6 g/kg BID) for 12 weeks, the femur and uterus of each animal were fixed and stained with H&E as described in Materials and methods. Representative photomicrographs from the sham, OVX and SHT groups. Arrow, growth plate; arrow head, trabeculae.
