HER2 overexpression is a putative diagnostic and prognostic biomarker for late-stage colorectal cancer in North African patients

Figures & data

Table 1. Clinico-pathological characteristics of the participants

Demographic and histopathological parameters	No.
Gender
Male	8
Female	8
Age
≤50 yr.	6
>50 yr.	11
Tumor subtype
Adenocarcinoma	15
Mucinous	2
Tumor grade
I	2
II	12
III	3
Tumor stage
T1-T2	6
T3-T4	11
Metastasis
Yes	3
No	14
HER2 IHC
Negative	9
Equivocal	4
Positive	4
Type of surgery
Right hemicolectomy (Rt HCE)	6
Left hemicolectomy (Lt HCE)	2
Low anterior resection (LAR)	2
Subtotal colectomy (SbCE)	1
Abdominoperineal resection (APR)	2

Table 2. Table of the HER2 IHC and qRT-PCR

	IHC score	IHC group	HER2	18S
			Ct mean	Ct mean	ΔCt
1	0	Negative	35.927	20.925	15.002
2	0	Negative	28.933	12.485	16.448
3	0	Negative	30.232	15.78	14.452
4	0	Negative	30.481	13.996	16.485
5	0	Negative	28.457	11.673	16.784
6	1	Negative	31.721	13.28	18.441
7	1	Negative	30.694	20.62	10.074
8	1	Negative	30.714	15.558	15.156
9	1	Negative	29.119	15.047	14.072
10	2	Equivocal	33.4	22.021	11.379
11	2	Equivocal	31.914	18.046	13.868
12	2	Equivocal	30.33	25.751	4.579
13	2	Equivocal	27.118	12.805	14.313
14	3	Positive	32.791	24.573	8.218
15	3	Positive	30.926	18.659	12.267
16	3	Positive	30.267	18.729	11.538
17	3	Positive	28.822	13.963	14.859

Figure 1. IHC staining images with (0, 1+, 2+, 3+) different scoring, were the 0, 1+ considered to be negative and 3+ considered to be positive

Figure 2. ERBB2 mRNA expression. Examples of real time PCR with SYBER Green. This was carried out in triplicate using RNA samples extracted from tumour cells microdissection from paraffin-embedded tissue sections

Figure 3. (a) Correlation of IHC group to HER2 protein expression and (b) correlation of HER2 mRNA expression between CRC study groups delta Ct mean to HER2 mRNA expression. * p < 0.05

Figure 4. In large cohort of 165 patients, high HER2 expression show a trend, after 40 months, towards poor overall survival in rectum adenocarcinoma