https://orcid.org/0000-0002-9741-5282
Figures & data
Table 1. Summary of proportions of sperm carrying X or Y chromosome, both X and Y chromosomes (X + Y) or neither X/Y chromosomes (∅) resulting from FISH analysis of testes and spermathecae post-copulation (left and middle column), and proportions of female and male progenies from Ag(PMB)1 and wild-type males crossed to wild-type G3 females (right column). Proportions of Y and X-bearing sperm and males and females are indicated as percentages and the total number of DAPI positive cells or adult mosquitoes counted are shown in parenthesis.
Figure 1. (A) Schematic representation of crosses to investigate the phenotypic effect of simultaneous meiotic cleavage of X and Y chromosome in A. gambiae. A. gambiae ASEMBO males were crossed to A. gambiae G3-derived Ag(PMB)1 females to generate YASXG3 (SD+) males carrying the autosomal insertion of the b2-tubulin::I-PpoI transgene and rDNA clusters on both sex chromosomes. The ASEMBO-derived rDNA cluster on the Y chromosome is expected to be smaller than the rDNA cluster in the X chromosome (Supplementary Figure 4). The I-PpoI nuclease is expected to cleave the 28S rDNA subunit present on both sex chromosomes during YASXG3(SD+) male meiosis. Consecutive crosses between YASXG3 (SD+) males and Ag(PMB)1 A. gambiae G3 females are expected to generate males carrying a Y chromosome previously exposed to I-PpoI cleavage (Y*AS, where ‘*’, ‘**’ ‘*[24]’ indicates I-PpoI exposure for 1, 2 or 24 consecutive generations) and a nonexposed X chromosome (XG3) chromosome. (B) Dot plots summarising the number of eggs laid per female and the number of larvae hatching from SD+ males (i and ii), and non-transgenic sibling males (iii and iv), carrying I-PpoI nonexposed (Y*AS or Y**AS) or exposed Y chromosomes (YAS) crossed to wild type females.. Wild-type males were also crossed as a control in each set of crosses. (C) Dot plots showing the mean percentage of male progeny from Ag(PMB)1, YASXG3(SD+), Y*ASXG3(SD+), Y**ASXG3(SD+), and Y*[24]ASXG3(SD+) males. Mean values shown in A and B are provided in Supplementary Table 1. P values indicate significance between crosses according to one-way ANOVA analysis on ranks for hatch rates). Horizontal bars indicate average values and error bars show 95% confidence intervals
![Figure 1. (A) Schematic representation of crosses to investigate the phenotypic effect of simultaneous meiotic cleavage of X and Y chromosome in A. gambiae. A. gambiae ASEMBO males were crossed to A. gambiae G3-derived Ag(PMB)1 females to generate YASXG3 (SD+) males carrying the autosomal insertion of the b2-tubulin::I-PpoI transgene and rDNA clusters on both sex chromosomes. The ASEMBO-derived rDNA cluster on the Y chromosome is expected to be smaller than the rDNA cluster in the X chromosome (Supplementary Figure 4). The I-PpoI nuclease is expected to cleave the 28S rDNA subunit present on both sex chromosomes during YASXG3(SD+) male meiosis. Consecutive crosses between YASXG3 (SD+) males and Ag(PMB)1 A. gambiae G3 females are expected to generate males carrying a Y chromosome previously exposed to I-PpoI cleavage (Y*AS, where ‘*’, ‘**’ ‘*[24]’ indicates I-PpoI exposure for 1, 2 or 24 consecutive generations) and a nonexposed X chromosome (XG3) chromosome. (B) Dot plots summarising the number of eggs laid per female and the number of larvae hatching from SD+ males (i and ii), and non-transgenic sibling males (iii and iv), carrying I-PpoI nonexposed (Y*AS or Y**AS) or exposed Y chromosomes (YAS) crossed to wild type females.. Wild-type males were also crossed as a control in each set of crosses. (C) Dot plots showing the mean percentage of male progeny from Ag(PMB)1, YASXG3(SD+), Y*ASXG3(SD+), Y**ASXG3(SD+), and Y*[24]ASXG3(SD+) males. Mean values shown in A and B are provided in Supplementary Table 1. P values indicate significance between crosses according to one-way ANOVA analysis on ranks for hatch rates). Horizontal bars indicate average values and error bars show 95% confidence intervals](/cms/asset/42d5fba6-2c16-438f-aed2-88f5414bf894/ypgh_a_1803628_f0001_b.gif)