Figures & data
Table 1. Primers.
Table 2. SbTF homologous proteins.
Table 3. HPLC gradient elution procedure for hypocrellin.
Figure 1. Phylogenetic tree of SbTF homologous proteins based on a maximum likelihood analysis of protein sequences. Maximum likelihood bootstrap support values (≥50%) are indicated above the nodes. The Shiraia-like strain zzz816 is shown in red, while other species with homologous transcription factors are shown in blue.
![Figure 1. Phylogenetic tree of SbTF homologous proteins based on a maximum likelihood analysis of protein sequences. Maximum likelihood bootstrap support values (≥50%) are indicated above the nodes. The Shiraia-like strain zzz816 is shown in red, while other species with homologous transcription factors are shown in blue.](/cms/asset/7efdeda6-b7c6-4127-b361-4840af5fbf7c/tmyc_a_2295406_f0001_oc.jpg)
Figure 4. Images of wild type strains and SbTF-overexpressing transformants cultured for 144 h on PDA plates. (a) Wild type CNUCC C72. (b) Transformant CNUCC C72. (c) Wild type zzz816. (d) Transformant zzz816.
![Figure 4. Images of wild type strains and SbTF-overexpressing transformants cultured for 144 h on PDA plates. (a) Wild type CNUCC C72. (b) Transformant CNUCC C72. (c) Wild type zzz816. (d) Transformant zzz816.](/cms/asset/c291d1da-a492-414a-8596-1e342821322c/tmyc_a_2295406_f0004_oc.jpg)
Figure 5. Comparison of wild type strains and SbTF-overexpressing transformants in liquid cultures. (a) Wild type CNUCC C72. (b) Transformant CNUCC C72. (c) Wild type zzz816 (low-yielding strain). (d) Wild type zzz816 (high-yielding strain). (e) Transformant zzz816.
![Figure 5. Comparison of wild type strains and SbTF-overexpressing transformants in liquid cultures. (a) Wild type CNUCC C72. (b) Transformant CNUCC C72. (c) Wild type zzz816 (low-yielding strain). (d) Wild type zzz816 (high-yielding strain). (e) Transformant zzz816.](/cms/asset/7fc79ee2-6c0b-4943-9c15-4634a04e5968/tmyc_a_2295406_f0005_oc.jpg)
Figure 8. Images of wild type strains and SbMNF-overexpressing transformants cultured for 144 h in PDB. (a) Wild type and transformant CNUCC 1353PR. (b) Wild type and transformant CNUCC C72. (c) Wild type and transformant zzz816.
![Figure 8. Images of wild type strains and SbMNF-overexpressing transformants cultured for 144 h in PDB. (a) Wild type and transformant CNUCC 1353PR. (b) Wild type and transformant CNUCC C72. (c) Wild type and transformant zzz816.](/cms/asset/92ce4458-bd57-4363-80b7-bc7d5ab69c3f/tmyc_a_2295406_f0008_oc.jpg)
Figure 9. Comparison of wild type strains and SbMNF-overexpressing transformant in liquid cultures. (a) Wild type CNUCC 1353PR. (b) Transformant CNUCC 1353PR. (c) Wild type CNUCC C72. (d) Transformant CNUCC C72. (e) Wild type zzz816 (low-yielding strain). (f) Wild type zzz816 (high-yielding strain). (g) Transformant zzz816.
![Figure 9. Comparison of wild type strains and SbMNF-overexpressing transformant in liquid cultures. (a) Wild type CNUCC 1353PR. (b) Transformant CNUCC 1353PR. (c) Wild type CNUCC C72. (d) Transformant CNUCC C72. (e) Wild type zzz816 (low-yielding strain). (f) Wild type zzz816 (high-yielding strain). (g) Transformant zzz816.](/cms/asset/c2641ef1-9488-48a3-b6ea-b45056a7b7c6/tmyc_a_2295406_f0009_oc.jpg)