Galleria mellonella as a model host for microbiological and toxin research

Figures & data

Table 1. Fungal species tested in G. mellonella.

Fungi	Assay	Reference
Aspergillus flavus	Virulence	^39
Aspergillus fumigatus	Virulence	^5,7
Aspergillus terreus	Virulence, antibiotic resistant and sensitive isolates	^40
Candida Africana	Virulence, assessing antifungal compounds	^41
Candida albicans	Biofilm formation, invasion, filamentation assay, virulence	^2,3,12,16,42,43
Candida glabrata	Virulence	^43
Candida krusei	Virulence, assessing antifungals compounds	^43,44
Candida metapsilosis	Virulence	^45
Candida orthopsilosis	Virulence	^45
Candida parapsilosis,	Virulence	^43,45
Candida tropicalis	Virulence, assessing antifungals compounds	^43,46
Conidiobolus coronatus (non-human pathogen)	Insect immunology, assessing fungal compunds	^47,48
Cryptococcus gattii	Virulence	^11
Cryptococcus neoformans	Virulence, host immune responses, assessing antifungal compounds	^4, 49,50
Fusarium cerealis	Virulence, assessing antifungal compounds	^9
Fusarium culmorum (non-human pathogen)	Virulence	^7
Fusarium oxysporum	Virulence	^7,10
Fusarium proliferatum (non-human pathogen)	Virulence	^7
Fusarium solani	Virulence	^7
Fusarium verticillioides	Virulence	^7
Histoplasma capsulatum	Virulence	^8
Madurella mycetomatis (non-human pathogen)	Induce grain formation	^51
Metarhizium robertsii (non-human pathogen)	Virulence	^52
Paracoccidioides brasiliensis	Virulence	^53
Paracoccidioides lutzii	Virulence	^8
Penicillium marneffei	Virulence, phagocytosis	^54
Pneumocystis murina	Virulence	^55
Rhizopus coincides	Virulence, thermotolerance	^56
Saccharomyces cerevisiae	Virulence	^2
Scedosporium aurantiacum	Virulence, substrate utilization	^57
Trichosporon asteroids	Virulence, Assessing antifungal compounds	^58
Trichosporon inkin	Virulence, Assessing antifungal compounds	^58
Trichosporon asahii	Virulence, Assessing antifungal compounds	^58

Table 2. Virus' tested in G. mellonella.

Virus	Assay	Reference
Bovine herpes simplex virus-1 (BHSV-1)	Nodulation	^59
Densonucleosis virus	Infectivity	^60
Invertebrate iridescent virus 6	Infectivity	^19
Iridovirus	Infectivity	^60
Mycovirus	Infectivity	^61
Nodamura virus	Infectivity, muscle cell modification	^62
Tipula iridescent virus TIV	Infectivity, gamma radiation responses	^18,63

Selvam RM, Nithya R, Devi PN, Shree RS, Nila MV, Demonte NL, Thangavel C, Maheshwari JJ, Lalitha P, Prajna NV, et al. Exoproteome of Aspergillus flavus corneal isolates and saprophytes: identification of proteoforms of an oversecreted alkaline protease. J Proteomics 2015; 115:23-35; PMID:25497218; http://dx.doi.org/10.1016/j.jprot.2014.11.017

 PubMed Web of Science ®Google Scholar

Maurer E, Browne N, Surlis C, Jukic E, Moser P, Kavanagh K, Lass-Flörl C, Binder U. Galleria mellonella as a host model to study Aspergillus terreus virulence and amphotericin B resistance. Virulence 2015; 6(6):591-8; PMID:26107350; http://dx.doi.org/10.1080/21505594.2015.1045183

 PubMed Web of Science ®Google Scholar

Borman AM, Szekely A, Linton CJ, Palmer MD, Brown P, Johnson EM. Epidemiology, antifungal susceptibility, and pathogenicity of Candida africana isolates from the United Kingdom. J Clin Microbiol 2013; 51(3):967-72; PMID:23303503; http://dx.doi.org/10.1128/JCM.02816-12

 PubMed Web of Science ®Google Scholar

Jacobsen ID. Galleria mellonella as a model host to study virulence of Candida. Virulence 2014; 5(2):237-9; PMID:24384470; http://dx.doi.org/10.4161/viru.27434

 PubMed Web of Science ®Google Scholar

Gago S, García-Rodas R, Cuesta I, Mellado E, Alastruey-Izquierdo A. Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis virulence in the non-conventional host Galleria mellonella. Virulence 2014; 5(2):278-85; PMID:24193303; http://dx.doi.org/10.4161/viru.26973

 PubMed Web of Science ®Google Scholar

Ligeza-Zuber M. Mechanisms of Galleria mellonella cellular immune response after infection with entomopathogenic fungus Conidiobolus coronatus. Ann Parasitol 2012; 58(4):227-8; PMID:23914619

 PubMedGoogle Scholar

Firacative C, Duan S, Meyer W. Galleria mellonella model identifies highly virulent strains among all major molecular types of Cryptococcus gattii. PLoS One 2014; 9(8):e105076; PMID:25133687; http://dx.doi.org/10.1371/journal.pone.0105076

 PubMedGoogle Scholar

Coleman JJ, Muhammed M, Kasperkovitz PV, Vyas JM, Mylonakis E. Fusarium pathogenesis investigated using Galleria mellonella as a heterologous host. Fungal Biol 2011; 115(12):1279-89; PMID:22115447; http://dx.doi.org/10.1016/j.funbio.2011.09.005

 PubMed Web of Science ®Google Scholar

Fallon JP, Troy N, Kavanagh K. Pre-exposure of Galleria mellonella larvae to different doses of Aspergillus fumigatus conidia causes differential activation of cellular and humoral immune responses. Virulence 2011; 2(5):413-21; PMID:21921688; http://dx.doi.org/10.4161/viru.2.5.17811

 PubMed Web of Science ®Google Scholar

Thomaz L, García-Rodas R, Guimarães AJ, Taborda CP, Zaragoza O, Nosanchuk JD. Galleria mellonella as a model host to study Paracoccidioides lutzii and Histoplasma capsulatum. Virulence 2013; 4(2):139-46; PMID:23302787; http://dx.doi.org/; http://dx.doi.org/10.4161/viru.23047

 PubMed Web of Science ®Google Scholar

Kloezen W, van Helvert-van Poppel M, Fahal AH, van de Sande WW. A Madurella mycetomatis Grain Model in Galleria mellonella Larvae. PLoS Negl Trop Dis 2015; 9(7):e0003926; PMID:26173126; http://dx.doi.org/10.1371/journal.pntd.0003926

 PubMed Web of Science ®Google Scholar

Giuliano Garisto Donzelli B, Krasnoff SB, Moon YS, Churchill AC, Gibson DM. Genetic basis of destruxin production in the entomopathogen Metarhizium robertsii. Curr Genet 2012; 58(2):105-16; PMID:22367459; http://dx.doi.org/10.1007/s00294-012-0368-4

 PubMed Web of Science ®Google Scholar

Scorzoni L, de Paula e Silva AC, Singulani Jde L, Leite FS, de Oliveira HC, da Silva RA, Fusco-Almeida AM, Mendes-Giannini MJ. Comparison of virulence between Paracoccidioides brasiliensis and Paracoccidioides lutzii using Galleria mellonella as a host model. Virulence 2015; 6(8):766-76; PMID:26552324; http://dx.doi.org/10.1080/21505594.2015.1085277

 PubMed Web of Science ®Google Scholar

Huang X, Li D, Xi L, Mylonakis E. Galleria mellonella Larvae as an Infection Model for Penicillium marneffei. Mycopathologia 2015; 180(3-4):159-64; PMID:26003722; http://dx.doi.org/10.1007/s11046-015-9897-y

 PubMed Web of Science ®Google Scholar

Fuchs BB, Bishop LR, Kovacs JA, Mylonakis E. Galleria mellonella are resistant to Pneumocystis murina infection. Mycopathologia 2011; 171(4):273-7; PMID:20922567; http://dx.doi.org/10.1007/s11046-010-9368-4

 PubMed Web of Science ®Google Scholar

Kaerger K, Schwartze VU, Dolatabadi S, Nyilasi I, Kovács SA, Binder U, Papp T, Hoog SD, Jacobsen ID, Voigt K. Adaptation to thermotolerance in Rhizopus coincides with virulence as revealed by avian and invertebrate infection models, phylogeny, physiological and metabolic flexibility. Virulence 2015; 6(4):395-403; PMID:26065324; http://dx.doi.org/10.1080/21505594.2015.1029219

 PubMed Web of Science ®Google Scholar

Cotter G, Doyle S, Kavanagh K. Development of an insect model for the in vivo pathogenicity testing of yeasts. FEMS Immunol Med Microbiol 2000; 27(2):163-9; PMID:10640612; http://dx.doi.org/10.1111/j.1574-695X.2000.tb01427.x

 PubMedGoogle Scholar

Kaur J, Duan SY, Vaas LA, Penesyan A, Meyer W, Paulsen IT, Nevalainen H. Phenotypic profiling of scedosporium aurantiacum, an opportunistic pathogen colonizing human lungs. PLoS One 2015; 10(3):e0122354; PMID:25811884; http://dx.doi.org/10.1371/journal.pone.0122354

 PubMedGoogle Scholar

Mariné M, Bom VL, de Castro PA, Winkelstroter LK, Ramalho LN, Brown NA, Goldman GH. The development of animal infection models and antifungal efficacy assays against clinical isolates of Trichosporon asahii, T. asteroides and T. inkin. Virulence 2015; 6(5):476-86; PMID:25751127; http://dx.doi.org/10.1080/21505594.2015.1020273

 PubMed Web of Science ®Google Scholar

Büyükgüzel E, Tunaz H, Stanley D, Büyükgüzel K. Eicosanoids mediate Galleria mellonella cellular immune response to viral infection. J Insect Physiol 2007; 53(1):99-105; PMID:17161422; http://dx.doi.org/10.1016/j.jinsphys.2006.10.012

 PubMed Web of Science ®Google Scholar

Buchatskiĭ LP, Litvinov GS, Lebedinets NN, Filenko OM, Podberezova LM. Vopr Virusol 1988; 33(5):603-6.

 PubMedGoogle Scholar

Constantino M, Christian P, Marina CF, Williams T. A comparison of techniques for detecting Invertebrate iridescent virus 6. J Virol Methods 2001; 98(2):109-18; PMID:11576637; http://dx.doi.org/10.1016/S0166-0934(01)00356-1

 PubMed Web of Science ®Google Scholar

Ozkan S, Coutts RH. Aspergillus fumigatus mycovirus causes mild hypervirulent effect on pathogenicity when tested on Galleria mellonella. Fungal Genet Biol 2015; 76:20-6; PMID:25626171; http://dx.doi.org/10.1016/j.fgb.2015.01.003

 PubMed Web of Science ®Google Scholar

Garzon S, Charpentier G, Kurstak E. Morphogenesis of the nodamura virus in the larbae of the lepidopteran Galleria mellonella (L.). Arch Virol 1978; 56(1-2):61-76; PMID:626593; http://dx.doi.org/10.1007/BF01317283

 PubMed Web of Science ®Google Scholar