Discovery of a mcr-1-bearing plasmid in commensal colistin-resistant Escherichia coli from healthy broilers in Faisalabad, Pakistan

Figures & data

Table 1. Characteristics of mcr-1-positive E. coli isolates from healthy broilers in Faisalabad, Pakistan.

Strains	Source	Date	MIC (μg/ml)	MLST	Farm no.
PK102	cloacal	27/12/2016	≥8	ST10	1
PK103	cloacal	27/12/2016	≥4	ST2847	1
PK105	cloacal	27/12/2016	≥8	ST155	1
PK107	cloacal	27/12/2016	≥8	New ST	1
PK109	cloacal	27/12/2016	≥4	ST361	1
PK110	cloacal	30/01/2017	≥4	ST6395	3
PK111	cloacal	30/01/2017	≥2	ST361	3
PK112	cloacal	30/01/2017	≥8	New ST	3

Figure 1. Genetic analyses of mcr-1-harboring plasmids in this study A. Scheme of different mcr-1-bearing plasmids B. PCR assays of mcr-1 and neighboring loci in plasmids 16S rDNA is specific to the E. coli specie.

Figure 2. The representative mcr-1-bearing plasmid in a colistin resistant E. coli isolate PK105 A. Diagram for the mcr-1-positive plasmid (pPK105) that exists in the E. coli isolate PK105 B. Colinear comparison of the IncI2-type plasmid pPK105 with two closely-related plasmids mcr-1-postive M18 and mcr-1-negative pColR644SK1.

Figure 3. MALDI-TOF MS analyses of lipid A pools of E. coli strains with (or without) mcr-1 A. MS profile of the LPS-lipid A in the negative control strain E. coli MG1655 B. MS spectrum of the LPS-lipid A in PK105, a representative strain of E. coli carrying mcr-1. A single modification may occur at the 1 (or 4′) position. The position indicated here is suggestive [20].

Xu Y, Lin J, Cui T, et al. Mechanistic insights into transferable polymyxin resistance among gut bacteria. J Biol Chem. 2018;293:4350–4365.

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