https://orcid.org/0000-0002-9505-8648
Figures & data
Figure 1. Confirmation of PPRV infection in lung, spleen, and cecum tissue of goats (a and b). Amplification of 351 bp N gene by RT-PCR. Lane M, 100 bp ladder; Lane 1, negative control; Lane 2, Infected goat lung; Lane 3, Infected goat spleen; Lane 4, Infected goat cecum.
Table 1. Distribution of lncRNAs as per class code
Figure 2. Overview of identification of lncRNAs, mRNAs, and neighboring genes of lncRNAs.
Figure 3. Genomic features of lncRNAs in PPRV infected and uninfected control lung and spleen tissues of goat. (a) Distribution of predicted lncRNAs as per class code. (b) Distribution of the number of exons in the predicted lncRNA transcripts (c) Chromosome-wise distribution of predicted lncRNA transcripts.
Table 2. Summary of transcript length of lncRNAs and their host/neighboring genes as obtained from LncEvo pipeline
Table 3. List of DElncRNAs from lung and spleen
Figure 4. Network of immune processes enriched for neighborhood genes whose expression was found to be highly correlated with DElncRNAs in the lung.
Figure 5. Network of immune processes enriched for neighborhood genes whose expression was found to be highly correlated with DElncRNAs in the spleen.
Figure 6. DElncRNA-selected neighboring genes regulatory network involved in immune processes. (a) Network of DElncRNAs (upregulated and downregulated) and the genes they regulate in the lung tissue. (b) Network of DElncRNAs (upregulated and downregulated) and the genes they regulate in the spleen tissue. DElncRNAs are represented by diamonds, with upregulated lncRNAs shown in red and downregulated lncRNAs in cyan. The neighboring genes are shown as ellipses, whose size is a function of their connectivity in protein-protein interactions (as shown in supplementary figure 2).
Figure 7. Gene Ontology analysis of differentially expressed genes. Functional enrichment analysis of DEmRNAs in lung and spleen showing immune pathways enriched in lung (a), immune pathways enriched in spleen (b), biological processes enriched in lung (c), and biological processes enriched in spleen (d) of PPRV infected goats.
