Clinical and genomic analysis of virulence-related genes in bloodstream infections caused by Acinetobacter baumannii

Figures & data

Table 1. Pertinent isolate characteristics.

Isolate	Patients’ gender	Patients’ age (year)	Source of bacteraemia	Antimicrobial phenotype	Hospital outcome	MLST	Growth rate (h^−1, 95% CI)	*Median survival (h)	Remarks
AB6	F	16	Wound	MDR	Alive	NT	1.779 (1.752–1.806)	>48	Control
AB8	M	72	Unknown	Non-MDR	Alive	457	1.547 (1.512–1.582)	>48	Control
AB23	M	74	Lung	Non-MDR	Expired	NT	1.860 (1.831–1.890)	6	-
AB26	F	67	Lung	MDR	Expired	NT	1.990 (1.953–2.026)	7	-
AB31	M	66	Lung	MDR	Alive	208	1.627 (1.600–1.653)	>24	Control

F-female; M-male; MDR – multidrug resistance, as nonsusceptibility to ≥ 1 agent in ≥ 3 antimicrobial categories; MLST – multilocus sequence typing; NT – Non-typable.

*In Galleria mellonella model (n = 10).

Figure 1. Venn diagram of different genes detected in all 5 isolates.

Pan genes describe the union of gene sequence entities that exist in all five isolates; core genes describe the gene sequences shared by all isolates. After the core genes are removed from the pan genes, the dispensable genes are obtained. Specific genes describe the gene sequences existing only within a particular isolate.

Table 2. Quality of DNA sequencing and genome information of A. baumannii isolates examined.

Isolate	Total Num (>500bp)	N50 Length (bp)	N90 Length (bp)	Sequence GC%	Genome size (bp)	Gene number (#)	Gene total length (bp)	Gene average length (bp)	Gene length/Genome (%)
AB6	145	54,969	15,612	39	3,844,440	3,716	3,314,676	892	86.2
AB8	53	147,699	42,862	38.95	3,880,200	3,714	3,403,065	916	87.7
AB23	56	168,007	42,864	38.95	3,918,936	3,720	3,415,293	918	87.2
AB26	60	158,175	42,707	38.95	3,923,171	3,724	3,424,644	920	87.3
AB31	57	143,736	50,558	39.09	4,148,595	4,004	3,614,154	903	87.1

Table 3. Potential genes associated with virulence identified.

VFDB internal ID	VF ID	VF name	Related genes
VFG005770	CVF171	Beta-hemolysin/cytolysin	acpC acyl carrier protein AcpC
VFG041842	SS016	Cpi-1a + Cpi-1 (SPI-1 like)	armR two-component response regulator
VFG010518	CVF348	Enhanced entry proteins	lidL TPR repeat protein
VFG001206	VF0272	FbpABC	fbpC ironIII ABC transporter, ATP-binding protein
VFG000839	VF0194	Paa	paa outer membrane adhesin Paa
VFG041142	SS193	T6SS	tssL/bscP putative type VI secretion system protein TssL
VFG041143	SS193	T6SS	tssK/bscO putative type VI secretion system protein TssK
VFG041146	SS193	T6SS	tssB/bscL putative type VI secretion system protein TssB
VFG041147	SS193	T6SS	tssC/bscK putative type VI secretion system protein TssC
VFG041148	SS193	T6SS	tssD/hcp/bscJ putative type VI secretion system protein TssD
VFG041149	SS193	T6SS	tssE/bscI putative type VI secretion system protein TssE
VFG041152	SS193	T6SS	tssH/clpV/bscF putative type VI secretion system protein TssH
VFG041154	SS193	T6SS	bscD putative outer membrane protein
VFG041156	SS193	T6SS	tssM/icmF/bscB putative type VI secretion system protein TssM
VFG041157	SS193	T6SS	bscA subfamily M15C metalopeptidase

VF – virulence factor; ID – identification.

Figure 2. Schematic illustration of the study processes.

Data availability statement

The data that support the findings of this study are openly available in National Center for Biotechnology Information (NCBI) at https://www.ncbi.nlm.nih.gov/, accession numbers: JAOAOM000000000, JAOAON000000000, JAOANQ000000000, JAOAOO000000000, and JAOBQI000000000, respectively.