Figures & data
Figure 1. Prenylation of Rab. Newly synthesized Rab associates with REP which forms a binary complex. Further, RGGTase binds to REP and forms the Rab-REP-RabGGTase ternary complex. RGGTase catalyze the transfer of prenyl groups to C-terminal cysteine residues. REP remain associated with prenylated Rab and targets it to the membrane.
Figure 2. Rab1 regulation by P. Legionella. 1) DrrA/SidM acts as GDF for Rab1 to displace GDI as well as acts as GEF to exchange GDP to GTP. 2) DrrA/SidM mediated AMPylation of GTP bound Rab1. 3) SidD mediated de-AMPylation of Rab1. 4) LepB functions as GAP, which leads to GTP hydrolysis and inactivation of Rab1. 5) AnkX mediated phosphocholination of Rab1 that renders it in an activated state.
Figure 3. Phosphoregulation of Rabs. Phosphorylation of GDP bound Rab inhibits its association with GDI which results in its failure to insert in target membrane. Dephosphorylation of Rab leads to its association with GDI, which takes it to the target membrane as well as its activation by GEF.
