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Brief Report

The anti-Rac1-GTP antibody and the detection of active Rac1: a tool with a fundamental flaw

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Pages 136-140 | Received 08 Mar 2021, Accepted 19 Apr 2021, Published online: 29 Apr 2021

Figures & data

Figure 1. Staining from the anti-Rac1-GTP antibody colocalizes with vimentin. Representative image of PC3 cells that have been fixed and stained for vimentin (green), DNA (blue), and with the anti-Rac1-GTP antibody (red). Scale bar, 10 μm.

Figure 1. Staining from the anti-Rac1-GTP antibody colocalizes with vimentin. Representative image of PC3 cells that have been fixed and stained for vimentin (green), DNA (blue), and with the anti-Rac1-GTP antibody (red). Scale bar, 10 μm.

Figure 2. Summary of the main methods available for the measurement of active Rac1.

Figure 2. Summary of the main methods available for the measurement of active Rac1.

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