https://orcid.org/0000-0002-0176-8906
Figures & data
Figure 1. p38 MAPK is activated ASK1-dependently in adipocytes
(a) Western blot (left panel) and quantified protein levels (right panel) of phosphorylated and total p38 MAPK in MEF-derived adipocytes isolated from ASK1F/F and ASK1∆adipo mice treated with or without recombinant TNFα. (b) Western blot and quantified protein levels of phosphorylated and total p38 MAPK in inguinal WAT of 12-week HFD-fed ASK1F/F (n = 11) and ASK1∆adipo (n = 15) mice. Values are expressed as mean ± SEM. *p < 0.05 (t-test), **p < 0.01 (two-way ANOVA).
Figure 2. Senescence markers are reduced in ASK1-deficient mice
(a) mRNA expression of Il-6 and Il-8 in inguinal WAT of 12-week HFD-fed ASK1F/F (n = 11–12) and ASK1∆adipo (n = 12–13) mice. (b) mRNA expression of p16Ink4α and p21 in inguinal WAT of 12-week HFD-fed ASK1F/F (n = 11–12) and ASK1∆adipo (n = 12–13) mice. (c) Western blot and quantified protein levels of p21 and p53 in isolated adipocytes of 12-week HFD-fed ASK1F/F (n = 6) and ASK1∆adipo (n = 6) mice. Values are expressed as mean ± SEM. #p = 0.08; *p < 0.05 (t-test).
Figure 3. LPS induces senescence markers ASK1-dependently in subcutaneous white adipocytes
mRNA expression of Il-6 (A), Il-8 (B), p16Ink4α (C) and p21 (D) in subcutaneous adipocytes transfected with control shRNA lentivirus (shLuc) or shRNA lentivirus targeting ASK1 (shASK1) pre-treated with or without 100 ng/ml LPS for 24 hours followed by stimulation with 0.1 µM isoproterenol for 6 hours. n = 7 (shLuc control), n = 8 (shASK1 control), n = 5 (shLuc LPS) or n = 7 (shASK1 LPS). Values are expressed as mean ± SEM. #p = 0.07 (for p21); #p = 0.05 (for Il-6); *p < 0.05; **p < 0.01 (two-way ANOVA).
