Figures & data
Figure 1. CD137 signaling induces IL-13, TNF and IL-6 secretion by HRS cell lines. 5 × 105 L-428-control, L-1236-control, L-428-CD137, L-1236-CD137 or KM-H2 cells were cultured on plates coated with 5 μg/mL of recombinant CD137L or BSA for 24 h. Levels of IL-13 (A), TNF (B) and IL-6 (C) were measured by ELISA. Depicted are means ± SD of triplicate measurements. *p <0.05; **p <0.01; ***p <0.005. Data are representative of three independent experiments.
Figure 2. (A) 5 × 105 L-1236-control or L-1236-CD137 cells were co-cultured with 5 × 105 THP-1 cells for 24 h. Supernatants from the cocultures were tested for IL-13 levels by ELISA. (B) PBMC were sub-optimally activated with 2 ng/mL of anti-CD3 (clone OKT3) and cultured in 50% conditioned supernatants of the cocultures of (A) for 24 h. 5 μg/mL of polyclonal goat IgG or goat polyclonal IL-13 antibody were added. (C) Anti-IL-13 antibody has been added at indicated concentrations to activated PBMC cultured in 50% conditioned supernatants of (A). IFNγ secretion by was measured by ELISA after 24 h. Depicted are means ± SD of triplicate measurements. *p <0.05; **p <0.01; ***p <0.005. Data are representative of three independent experiments.
Figure 3. CD137 expression increases HRS cell proliferation. Indicated numbers of L-428 (A) or L-1236 (B) or KM-H2 (C) cells were seeded (white bar: non-CD137-expressing cells, black bar: CD137-expressing cells), and pulsed with 0.5 μCi of 3H-thymidine at the same time. The cells were harvested 24 h later, and 3H-thymidine incorporation was quantified. Depicted are means ± SD. *p <0.05, **p <0.01, ***p < 0.001.
Figure 5. Schematic representation of the role of CD137 in immune deviation and growth stimulation of HRS cells.
Table 1. Expression of CD137 and IL-13 in HL tumor microarray determined by immunohistochemical double staining. For CD137: + at least 5 cells, ++ at least 10 cells, +++ more than 15 cells stained in the section. For IL-13 staining: + at least 10%, ++ at least 30% and +++ more than 50% of the section stained.
Figure 4. CD137-positive cells in HL express IL-13. Immunohistochemical detection of IL-13 (green) and CD137 (red). Shown are three cases of classical HL of the mixed cellularity type. Size bar: 10 μm.
