Biosynthesis of ethyl (S)-4-chloro-3-hydroxybutanoate with an NADH-dependent reductase (ClCR) discovered by genome data mining using a modified colorimetric screening strategy

Figures & data

Figure 1. Proposed color-generating mechanism (a). Effects of asorbance wavelength (b), FeCl₃ concentration (c), incubation temperature (d), and incubation time (e) on color-developing.

Figure 2. SDS–PAGE analysis of recombinant protein of ClCR. Line 1: Marker. Line 2: supernatant (soluble proteins) after sonication of E. coli CCZU-T15.

Figure 3. Effects of the organic solvent (a) and potential cosubstrate (b) on the reductase activity.

Figure 4. Time courses for the biotransformation of COBE into (S)-CHBE. 50–200 mM COBE in the monophasic aqueous media; 500–1000 mM COBE in the biphasic media.