Engineering the glycolytic pathway: A potential approach for improvement of biocatalyst performance

Figures & data

Figure 1. Glycolysis via the EMP and ED pathways. Names of enzymes are underlined. Abbreviations: 6PGD, gluconate 6-phosphate dehydratase; 6PGL, 6-phosphogluconolactonase; ENO, enolase; FBA, fructose 1,6-bisphosphate aldolase; G6PDH, glucose 6-phosphate dehydrogenase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GLK, glucokinase; GPI, glucose 6-phosphate isomerase; KDPGA, 2-keto-3-deoxy-6-phosphogluconate aldolase; PFK, 6-phosphofructokinase; PGK, phosphoglycerate kinase; PGM, phosphoglycerate mutase; PTS, phosphoenolpyruvate:carbohydrate phosphotransferase system; PYK, pyruvate kinase; TPI, triosephosphate isomerase; DHAP, dihydroxyacetone phosphate; GAP, glyceraldehyde 3-phosphate; KDPG, 2-keto-3-deoxy-6-phosphogluconate.

Table 1. Improvement of productivity by engineering of the glycolytic pathway.

Organism	Overproduced enzyme	Product	Productivity improvement (fold)^*	Reference
C. glutamicum	GAPDH	Alanine	2.7	16
	GPI, PFK, GAPDH, PYK	Alanine	6.4	17
	GPI, PFK, TPI, GAPDH, PYK	L-Valine	9	19
	GPI, PFK, GAPDH, PYK	Isobutanol	1.8	22
	GLK	D-Lactate	1.8	24
	GAPDH	D-Lactate	1.5	24
	GLK, GAPDH, PFK, FBA, TPI	D-Lactate	2.5	25
	GPI, PFK, TPI, GAPDH, PYK	Ethanol	2.1	21
E. coli	GAPDH	1-Butanol	1.5	45
	PFK, PYK	Ethanol	1.4	31
L. lactis	PFK	Lactate	2.2	29
Z. mobilis	G6PDH	Ethanol	1.1	34
	GLK	Ethanol	1.1	34
S. cerevisiae	PFK	Ethanol	1.3	32

* Relative productivity compared to that of control strain.

Abbreviations are listed in Fig. 1.