Regulatory roles of 24-epibrassinolide in tolerance of Acacia gerrardii Benth to salt stress

Figures & data

Table 1. Effect of NaCl (200 mM) on morphological criteria (shoot height(SH) [cm], root depth(RD) [cm], shoot dry weight (DW) [g] of and rootDW[g] A.gerrardii treated with and without 24-epibrassinolide. Data presented is the mean of 10 replicates.

	Morphological criteria
	Shoot		Root	SH/RD
Treatments	Height	DW	Depth	DW	SDW/RDW
Control	47.1 ± 2.11b	1.6 ± 0.14b	45.7 ± 2.07b	1.26 ± 0.07b	1.03 ± 0.04ab	1.28 ± 0.01b
EBL	64.7 ± 2.64a	2.5 ± 0.18a	61.2 ± 2.81a	1.99 ± 0.12a	1.05 ± 0.04ab	1.27 ± 0.01b
NaCl	18.1 ± 1.72d	0.5 ± 0.07d	19.3 ± 1.42d	0.38 ± 0.02d	0.93 ± 0.03c	1.38 ± 0.02ab
NaCl + EBL	33.8 ± 1.96c	1.2 ± 0.11c	29.4 ± 1.68c	0.87 ± 0.06c	1.15 ± 0.04a	1.46 ± 0.06a
LSD at: 0.05	3.78	0.23	4.82	0.15	0.01	0.01

Table 2. Effect of NaCl (200 mM) on chlorophyll a (Chl a), chlorophyll b (Chl b), carotenoids (Carot) and total chlorophyll content (mg g⁻¹ FW) in A.gerrardiiBenth treated with and without 24-epibrassinolide. Data presented is the mean of 10 replicates.

	Photosynthetic pigments (mg g fresh weight^−1)
Treatments	Chl a	Chl b	A+B	A/B	Carot	Total pigments
Control	1.69 ± 0.18b	0.47 ± 0.03b	2.17 ± 0.11b	3.56 ± 0.12	0.19 ± 0.01b	2.36 ± 0.06b
EBL	2.05 ± 0.24a	0.61 ± 0.04a	2.66 ± 0.11a	3.34 ± 0.12	0.29 ± 0.3a	2.95 ± 0.05a
NaCl	0.77 ± 0.08d	0.17 ± 0.02d	0.95 ± 0.05d	4.59 ± 0.27	0.06 ± 0.01d	1.01 ± 0.03d
NaCl + EBL	1.22 ± 0.16c	0.33 ± 0.03c	1.55 ± 0.16c	3.64 ± 0.23	0.12 ± 0.01c	1.67 ± 0.04c
LSD at: 0.05	0.27	0.11	0.35	0.07	0.02	0.42

Table 3. Effect of NaCl (200 mM) on relative water content (RWC, %), stomatal conductance (gs, molm⁻² s⁻¹), transpiration rate (E, mmol H₂O⁻² S⁻¹) and membrane stability index (MSI, %) in A.gerrardii Benth treated with and without 24-epibrassinolide. Data presented is mean of 10 replicates.

Treatments	Relative water content RWC (%)	Stomatal conductance(gs)	Transpiration rate(E)	Membrane stability index(MSI)
Control	84.5 ± 2.4b	0.32 ± 0.07b	5.96 ± 0.31b	85.9 ± 1.7b
EBL	90.6 ± 2.5a	0.52 ± 0.09a	6.92 ± 0.46a	94.4 ± 1.9a
NaCl	67.3 ± 1.9d	0.15 ± 0.02d	3.06 ± 0.25d	48.8 ± 1.1c
NaCl + EBL	76.4 ± 3.0c	0.26 ± 0.03c	5.08 ± 0.32bc	80.1 ± 1.5b
LSD at: 0.05	5.12	0.14	0.53	3.47

Figure 1. Effect of NaCl (200 mM) on nitrate reductase activity in Acacia gerrardii Benth treated with 24-epibrassinolide. Data present is mean of 10 replicates.

Figure 2. Effect of NaCl (200 mM) on superoxide dismutase (A), catalase (B), ascorbate peroxidase (C), glutathione reductase (D), dehydroascorbate reductase (E) and monodehydroascorbate reductase (F) activity in Acacia gerrardii Benth treated with 24-epibrassinolide. Data present is mean of 10 replicates.

Yusuf, M., Fariduddin, Q., Ahmad. A. 24-Epibrassinolide modulates growth, nodulation, antioxidant system, and osmolyte in tolerant and sensitive varieties of Vigna radiata under different levels of nickel: A shotgun approach. Plant Physiol. Biochem 2012; 57:143-153; PMID:22705589; https://doi.org/10.1016/j.plaphy.2012.05.004

 Google Scholar

Abd_Allah EF, Hashem A, Alqarawi AA, Bahkali AH, Alwhibi MS. Enhancing growth performance and systemic acquired resistance of medicinal plant Sesbania sesban (L.) Merr using arbuscular mycorrhizal fungi under salt stress. Saudi J. Biol. Sci 2015; 22:274-83; PMID:25972748; https://doi.org/10.1016/j.sjbs.2015.03.004

 Google Scholar