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Bioengineered Volume 12, 2021 - Issue 1
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Special issue on Biotechnology-Driven Innovations Towards Sustainable Development

Optimization of culture conditions for gamma-aminobutyric acid production by newly identified Pediococcus pentosaceus MN12 isolated from ‘mam nem’, a fermented fish sauce

Do Thi Bich Thuya Hue University of Agriculture and Forestry, Hue University, Hue, VietnamCorrespondence[email protected]
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An Tien Nguyenb Faculty of Agriculture and Forestry, Dalat University, Dalat, VietnamORCID Iconhttps://orcid.org/0000-0002-5603-3667View further author information
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Kuan Shiong Khooc Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Selangor Darul Ehsan, MalaysiaORCID Iconhttps://orcid.org/0000-0002-5369-2675View further author information
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Kit Wayne Chewd School of Energy and Chemical Engineering, Xiamen University Malaysia, Jalan Sunsuria, Bandar Sunsuria, Sepang, Selangor, MalaysiaView further author information
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Margo Cnockaerte Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, BelgiumView further author information
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Peter Vandammee Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, BelgiumView further author information
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Yeek-Chia Hof Civil and Environmental Engineering Department, Universiti Teknologi PETRONAS, Seri Iskandar, Perak Darul Ridzuan, Malaysia;g Centre for Urban Resource Sustainability, Institute of Self-Sustainable Building, Universiti Teknologi PETRONAS, Perak Darul Ridzuan, MalaysiaORCID Iconhttps://orcid.org/0000-0002-2820-9696View further author information
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Nguyen Duc Huyh Institute of Biotechnology, Hue University, Hue, VietnamView further author information
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Heriberto Hernández Cocoletzii Facultad de Ingeniería Química, Benemérita Universidad Autónoma de Puebla, Pue, MéxicoView further author information
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Pau Loke Showc Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Selangor Darul Ehsan, MalaysiaORCID Iconhttps://orcid.org/0000-0002-0913-5409View further author information
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Pages 54-62 | Received 14 Aug 2020, Accepted 25 Nov 2020, Published online: 22 Dec 2020

Figures & data

Figure 1. GABA-producing capability of different LAB isolates from mam nem. Cells were grown with initial cell density of 107 CFU/mL in MRS broth supplemented with 60 mM of MSG for 24 h at 37°C. Concentration of GABA in culture supernatants were quantified by an HPLC method. Data are means ± SD of GABA production from triplicate experiments. Bars without a common letter differ significantly (P < 0.05)

Figure 1. GABA-producing capability of different LAB isolates from mam nem. Cells were grown with initial cell density of 107 CFU/mL in MRS broth supplemented with 60 mM of MSG for 24 h at 37°C. Concentration of GABA in culture supernatants were quantified by an HPLC method. Data are means ± SD of GABA production from triplicate experiments. Bars without a common letter differ significantly (P < 0.05)

Figure 2. Effect of initial cell density on the GABA production of P. pentosaceus MN12. Cells were grown in MRS broth supplemented with 60 mM of MSG at 37°C for 24 h. Concentration of GABA in culture supernatants were quantified by an HPLC method. Data are means ± SD of GABA production from triplicate experiments. Bars without a common letter differ significantly (P < 0.05)

Figure 2. Effect of initial cell density on the GABA production of P. pentosaceus MN12. Cells were grown in MRS broth supplemented with 60 mM of MSG at 37°C for 24 h. Concentration of GABA in culture supernatants were quantified by an HPLC method. Data are means ± SD of GABA production from triplicate experiments. Bars without a common letter differ significantly (P < 0.05)

Figure 3. Effect of monosodium glutamate (MSG) concentration on the GABA production of P. pentosaceus MN12. Cells were grown in MRS broth with initial cell density of 5.106 CFU/mL at 37°C for 24 h. Concentration of GABA in culture supernatants were quantified by an HPLC method. Data are means ± SD of GABA production from triplicate experiments. Bars without a common letter differ significantly (P < 0.05)

Figure 3. Effect of monosodium glutamate (MSG) concentration on the GABA production of P. pentosaceus MN12. Cells were grown in MRS broth with initial cell density of 5.106 CFU/mL at 37°C for 24 h. Concentration of GABA in culture supernatants were quantified by an HPLC method. Data are means ± SD of GABA production from triplicate experiments. Bars without a common letter differ significantly (P < 0.05)

Figure 4. Effect of initial pH on the GABA production of P. pentosaceus MN12. Cells were grown in MRS broth with initial cell density of 5.106 CFU/mL at 37°C for 24 h. Concentration of GABA in culture supernatants were quantified by an HPLC method. Data are means ± SD of GABA production from triplicate experiments. Bars without a common letter differ significantly (P < 0.05)

Figure 4. Effect of initial pH on the GABA production of P. pentosaceus MN12. Cells were grown in MRS broth with initial cell density of 5.106 CFU/mL at 37°C for 24 h. Concentration of GABA in culture supernatants were quantified by an HPLC method. Data are means ± SD of GABA production from triplicate experiments. Bars without a common letter differ significantly (P < 0.05)

Figure 5. Effect of temperature on the GABA production from P. pentosaceus MN12. Cells were grown in MRS broth with initial cell density of 5.106 CFU/mL at initial pH 7 for 24 h. Concentration of GABA in culture supernatants were quantified by an HPLC method. Data are means ± SD of GABA production from triplicate experiments. Bars without a common letter differ significantly (P < 0.05)

Figure 5. Effect of temperature on the GABA production from P. pentosaceus MN12. Cells were grown in MRS broth with initial cell density of 5.106 CFU/mL at initial pH 7 for 24 h. Concentration of GABA in culture supernatants were quantified by an HPLC method. Data are means ± SD of GABA production from triplicate experiments. Bars without a common letter differ significantly (P < 0.05)

Figure 6. Effect of fermentation time on pH (a), cell growth (b) and GABA accumulation (c) by P. pentosaceus MN12. Cells were grown in MRS broth in optimal conditions for 120 h. The measurements of pH, cell growth and GABA content were taken at 24 h intervals. Data are means ± SD from triplicate experiments. Means without a common letter differ significantly (P < 0.05)

Figure 6. Effect of fermentation time on pH (a), cell growth (b) and GABA accumulation (c) by P. pentosaceus MN12. Cells were grown in MRS broth in optimal conditions for 120 h. The measurements of pH, cell growth and GABA content were taken at 24 h intervals. Data are means ± SD from triplicate experiments. Means without a common letter differ significantly (P < 0.05)

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