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Bioengineered Volume 12, 2021 - Issue 1
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research paper

Homebrewed psilocybin: can new routes for pharmaceutical psilocybin production enable recreational use?

William J. Gibbons Jr.a Department of Chemical, Paper, and Biomedical Engineering, Miami University, Oxford, OH, USAView further author information
,
Madeline G. McKinneya Department of Chemical, Paper, and Biomedical Engineering, Miami University, Oxford, OH, USAView further author information
,
Philip J. O’Dellb Department of Chemistry and Biochemistry, Miami University, Oxford, OH, USAView further author information
,
Brooke A. Bollingera Department of Chemical, Paper, and Biomedical Engineering, Miami University, Oxford, OH, USAView further author information
&
J. Andrew Jonesa Department of Chemical, Paper, and Biomedical Engineering, Miami University, Oxford, OH, USACorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0002-9068-2126View further author information
ORCID Icon
Pages 8863-8871 | Received 07 Aug 2021, Accepted 24 Sep 2021, Published online: 26 Oct 2021

Figures & data

Figure 1. Visual representation of the homebrew experimental setup. Two 500 mL bottles, containing inoculated cultures, magnetic stir bars, and air stones, were placed into a plastic tub filled with water. The air stones were attached to small aquarium air pumps via plastic tubing. The tops of the bottles were covered with aluminum foil. The tub was placed onto two magnetic stir plates, set to 510 RPM. A sous vide, set to 37 °C, was attached to the plastic tub and inserted into the water bath

Figure 1. Visual representation of the homebrew experimental setup. Two 500 mL bottles, containing inoculated cultures, magnetic stir bars, and air stones, were placed into a plastic tub filled with water. The air stones were attached to small aquarium air pumps via plastic tubing. The tops of the bottles were covered with aluminum foil. The tub was placed onto two magnetic stir plates, set to 510 RPM. A sous vide, set to 37 °C, was attached to the plastic tub and inserted into the water bath

Figure 2. Psilocybin titers under each set of culture conditions. * denotes significant difference, p < 0.01; n.s. denotes no significant difference, p > 0.1

Figure 2. Psilocybin titers under each set of culture conditions. * denotes significant difference, p < 0.01; n.s. denotes no significant difference, p > 0.1

Figure 3. Flow chart illustrating possible regulatory points

Figure 3. Flow chart illustrating possible regulatory points
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