671
Views
1
CrossRef citations to date
0
Altmetric
Research Paper

Inhibitory activity of Halobacillus trueperi S61 and its active extracts on potato dry rot

, &
Pages 3852-3867 | Received 26 Nov 2021, Accepted 27 Dec 2021, Published online: 15 Feb 2022

Figures & data

Figure 1. Morphological identification of Qing 9A-1-1 (a) on medium and (b) microscope (400), Qing 9A-2 (c) on medium and (d) microscope (400), and Qing A-5-8 (e) on medium and (f) microscope (400).

Figure 1. Morphological identification of Qing 9A-1-1 (a) on medium and (b) microscope (400), Qing 9A-2 (c) on medium and (d) microscope (400), and Qing A-5-8 (e) on medium and (f) microscope (400).

Figure 2. Phylogenetic tree based on sequences (a) Qing A-5-8, (b) Qing 9A-2 and (c) Qing 9A-1-1.

Figure 2. Phylogenetic tree based on sequences (a) Qing A-5-8, (b) Qing 9A-2 and (c) Qing 9A-1-1.

Table 1. Effect of single and combine infection on three potato varieties

Figure 3. Pathogenicity effect of pathogens on potato in vivo. A was infection effect of pathogen on Qingshu 9, B was infection effect of pathogen on Qingshu 2, and C was infection effect of pathogen on Xiazhai 65. From left to right were infected by sterile water, Qing 9A-2, Qing 9A-1-1, Qing 9A-2 combine Qing 9A-1-1.

Figure 3. Pathogenicity effect of pathogens on potato in vivo. A was infection effect of pathogen on Qingshu 9, B was infection effect of pathogen on Qingshu 2, and C was infection effect of pathogen on Xiazhai 65. From left to right were infected by sterile water, Qing 9A-2, Qing 9A-1-1, Qing 9A-2 combine Qing 9A-1-1.

Table 2. Infection rate of single infection on three potato varieties

Table 3. Inhibitory activity of strain S61 and its ethyl acetate extract on pathogens

Figure 4. Inhibition of strain S61 (a) Qing 9A-2, (b) Qing 9A-1-1 and (c) Qing 9A-5-8 on three pathogenic bacteria in vitro, as well as confrontation result of Qing 9A-2 and S61 (d), Qing 9A-5-8 and S61 (e), Qing 9A-1-1 and S61 (f) on the medium.

Figure 4. Inhibition of strain S61 (a) Qing 9A-2, (b) Qing 9A-1-1 and (c) Qing 9A-5-8 on three pathogenic bacteria in vitro, as well as confrontation result of Qing 9A-2 and S61 (d), Qing 9A-5-8 and S61 (e), Qing 9A-1-1 and S61 (f) on the medium.

Table 4. Control effect of spraying s61 suspension and its ethyl acetate extract

Figure 5. Electron microscopic observation of S61 on pathogens (a) Qing 9A-2, (b) Qing 9A-1-1 and (c) Qing A-5-8, as well as the in vivo effect of spraying S61 (d) and its ethyl acetate extract (e) on Qing 9A-2, and colonization of S61 on potato tubers (f).

Figure 5. Electron microscopic observation of S61 on pathogens (a) Qing 9A-2, (b) Qing 9A-1-1 and (c) Qing A-5-8, as well as the in vivo effect of spraying S61 (d) and its ethyl acetate extract (e) on Qing 9A-2, and colonization of S61 on potato tubers (f).

Figure 6. Response of enzymatic activity after sprayed strain S61 and its extract: peroxidase (POD) after spraying S61 (a) and its ethyl acetate extract (b), oxidase (PPO) after spraying S61 (c) and ethyl acetate extract (d), superoxide dismutase (SOD) after spraying S61 (e) and ethyl acetate extract (f), catalase (CAT) after spraying S61 (g) and ethyl acetate extract (h).

Figure 6. Response of enzymatic activity after sprayed strain S61 and its extract: peroxidase (POD) after spraying S61 (a) and its ethyl acetate extract (b), oxidase (PPO) after spraying S61 (c) and ethyl acetate extract (d), superoxide dismutase (SOD) after spraying S61 (e) and ethyl acetate extract (f), catalase (CAT) after spraying S61 (g) and ethyl acetate extract (h).