https://orcid.org/0000-0002-7595-4103
Figures & data
Table 1. The primer sequence in the study
Figure 1. LncRNA TRPM2-AS was overexpressed in esophageal cancer (EC) tissues and cell lines.
(A) lncRNA TRPM2-AS expression was determined in 15 paired EC and adjacent normal tissues using quantitative real-time polymerase-chain reaction (qRT-PCR). (B) LncRNA TRPM2-AS expression was determined in human EC cell lines (KYSE-520 cells and ECA-109 cells) and human normal esophageal epithelial cells (HEEC) using qRT-PCR. *p < 0.05, ** p < 0.01, n = 3.
Figure 2. Silencing of lncRNA TRPM2-AS suppresses cell proliferation in esophageal cancer (EC) cells.
(A) The knockdown efficiency was determined by quantitative real-time polymerase-chain reaction in EC cell lines after transfecting with si-TRPM2-AS and si-NC for 48 h, respectively. (B) Cell proliferation at indicated timepoints was determined by cell counting kit-8 assay in EC cell lines after silencing of lncRNA TRPM2-AS. (C) Colony formation assay results of KYSE-520 cells and ECA-109 cells. Colony formation assays were carried out 2 weeks after silencing of lncRNA TRPM2-AS. **p < 0.01, ***p < 0.001, n = 3.
Figure 3. Silencing of lncRNA TRPM2-AS promotes cell apoptosis in esophageal cancer cells.
(A) Presentative images of flow cytometry assay were shown. Flow cytometry was carried out after 48 h post si-TRPM2-AS or si-NC transfection in KYSE-520 cells. (B) Flow cytometry was carried out after 48 h post si-TRPM2-AS or si-NC transfection in ECA-109 cells. ** p < 0.01, n = 3.
Figure 4. Silencing of lncRNA TRPM2-AS inhibits cell migration and invasion in esophageal cancer cells.
(A) Cell migration and invasion results of KYSE-520 cells determined at 48 h post cell seeding in the transwell chambers. (B) Cell migration and invasion results of ECA-109 cells determined at 48 h post cell seeding in the transwell chambers. **p < 0.01, n = 3.
Figure 5. LncRNA TRPM2-AS knockdown suppresses the epithelial–mesenchymal transitions (EMT) progress in esophageal cancer cells.
(A) EMT-related genes expressions were determined by quantitative real-time polymerase-chain reaction in KYSE-520 cells and ECA-109 cells after lncRNA TRPM2-AS knockdown. (B) Epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), Vimentin, and matrix metallopeptidase 9 (MMP-9) protein expressions were determined by Western blotting in KYSE-520 and ECA-109 cells after lncRNA TRPM2-AS knockdown. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n = 3.
Figure 6. LncRNA TRPM2-AS regulates miR-1291, miR-6852-5p, and miR-138-5p expression in esophageal cancer cells.
(A) The interaction network between lncTRPM2-AS and potential targeting miRNAs was constructed using cytoscape. (B) The expression of miR-1291, miR-6852-5p, miR-138-5p, miR-218-5p, and miR-93-3p were determined using quantitative real-time polymerase-chain reaction in KYSE-520 cells and ECA-109 cells after lncRNA TRPM2-AS knockdown. NS: no significance; **p < 0.01, n = 3.
Supplemental material
Supplemental Material
Download MS Excel (18.8 KB)Data availability statement
All Data are showed in this study.
