Figures & data
Table 1. The theoretical affinity of quercitrin with respect to GJB2
Figure 1. Quercitrin reduces LUAD cell growth, migration, and invasion. a-b. CCK-8 proliferation assay of H1299 and H1650 cells treated with different concentrations of quercitrin for 24 hours, 48 hours, or 72 h. c. Colony formation assay of H1299 and H1650 cells treated with quercitrin (2, 5, 10 μM). d. Wound healing assay of H1299 and H1650 cells treated with quercitrin. E. Transwell invasion assays showed that quercitrin decreased the invasion ability of H1299 and H1650 cells. Data represent the Mean ± SD for at least three independent experiments. *P< 0.05, **P< 0.01 compared with control.
Figure 2. Differentially expressed genes in LUAD. a. Heat maps of dysregulated genes in GSE18842 and GSE19804 databases. b. Venn diagram of dysregulated genes in common in the two datasets. c. The molecular docking model of quercitrin with GJB2. d. The expression profiling of GJB2 in LUAD tissue and normal tissue. e. Patient populations were split into high expression and low expression groups by median value (Cutoff value = 418). The higher expression level of GJB2 was associated with worse overall survival in patients with LUAD.
Figure 3. Deletion of GJB2 inhibits LUAD cells migration and invasion. a. H1299 and H1650 were transfected with si-GJB2 (#1 or #2). The expression of GJB2 was determined by Western blot. b. H1299 and H1650 were transfected with si-GJB2 (#1 or #2). The growth of LUAD cells was evaluated with a colony formation assay. c. Wound healing assay of GJB2 silencing H1299 and H1650 cell. d. Transwell invasion assay of GJB2 silencing H1299 and H1650 cell. Data represent the Mean ± SD for at least three independent experiments. **P< 0.01 compared with si-Con.
Figure 4. GJB2 expression is reduced by quercitrin. a. GJB2 expressions in quercitrin-treated H1299 and H1650 cells were determined by Western blot. b. The protein expression of GJB2 was determined by immunofluorescence. c. The mRNA levels of GJB2 in LUAD cells were determined using qRT-PCR. **P< 0.01 compared with control.
Figure 5. GJB2 is required for the ai-cancer effect of quercitrin in LUAD cell. a. GJB2 expressions in H1299 and H1650 cells transfected with GJB2-OE were determined by Western blot. b. Colony formation assay showed that the growth of H1299 and H1650 cells was increased after GJB2 overexpression in the presence of quercitrin. c-d. Wound healing and Transwell invasion assay showed that the migration and invasion abilities of H1299 and H1650 cells were increased after GJB2 transfection. Data represent the Mean ± SD for at least three independent experiments. **P< 0.01 compared with control, ##P< 0.01 compared with quercitrin.
