Figures & data
Table 1. The sequence of primers
Table 2. Comparison of miRNA-146, E2, FSH and ROS levels between the two groups in POI model (x¯±s)
Table 3. Comparison of miRNA-146, E2, FSH and ROS levels between the two groups by injection of miRNA-146 mimics in POI model (x¯±s)
Figure 1. LPS suppresses viability of granulosa cells.
Figure 2. Transfection efficiency of miRNA-146 in granulosa cells. a: miNA-146 mimics, inhibitors and transfection efficiency. The expression levels of miRNA-146 in granulosa cells and negative control group were detected by PCR. GAPDH is quality control, *** P < 0.001.
Figure 3. The levels of miRNA-146 expression and IL-6, and TNF-a in granulosa cells. C: Granulosa cells were transfected using miR-146 mimics, miRNA-146 inhibitors or negative control (NC). The effect of miRA-146 on siTLR4 expression in granulosa cells was measured by qRT PCR and Western blot. *P < 0.01.
Figure 4. The levels of Bcl-2 and Bax protein were analyzed by western blotting. A) Overexpression of miR-146 repress apoptosis of OGCs by increasing Bcl-2, ** P < 0.01, * P < 0.05, B) versus the control. C) Overexpression of miR-146 inhibited apoptosis of OGCs by reducing Bax, ***P < 0.001, ** P < 0.01, * P < 0.05, versus the control.
Figure 5. The levels of miR-146 expression and TLR4 and NF-ĸB expression. a: TLR4 expression. These granulosa cells were transfected with miR-146 mimics, miR-146 inhibitors, control, or TLR4 siRNA (si-TLR4), and were treated using concentration of 10 μg/mL LPS for 12 h by qPCR. * P < 0.05; ** P < 0.01; *** P < 0.001. B: NF-ĸB expression. These granulosa cells were transfected with miR-146 mimics, miR-146 inhibitors, control, or TLR4 siRNA (si-TLR4), and were treated using concentration of 10 μg/mL LPS for 12 h by qPCR. * P < 0.05; ** P < 0.01; *** P < 0.001. C: The levels of TLR4 and NF-ĸB expression in granulosa cells were detected by western blot analysis.
