Figures & data
Schematic illustration for the preparation of Bio-PA/EPI NPs using dialysis method and establishment of tumor model in vivo and in vitro.
![Schematic illustration for the preparation of Bio-PA/EPI NPs using dialysis method and establishment of tumor model in vivo and in vitro.](/cms/asset/1f6ceea0-3a60-4fcb-972a-25009b3add14/ianb_a_1544142_uf0001_c.jpg)
Figure 4. Morphological changes of HepG2 cells in defferent culture model: 2D (a), 3D (b) imaged by invert microscope. The balling rate (%) of two cell culture methods (c).
![Figure 4. Morphological changes of HepG2 cells in defferent culture model: 2D (a), 3D (b) imaged by invert microscope. The balling rate (%) of two cell culture methods (c).](/cms/asset/d11291de-dad8-4e38-877a-d655499d6b54/ianb_a_1544142_f0004_c.jpg)
Figure 5. Confocal image of 2D in monolayer, 3D in spheroids and frozen section of tumor tissue (scale bar is 100 µm).
![Figure 5. Confocal image of 2D in monolayer, 3D in spheroids and frozen section of tumor tissue (scale bar is 100 µm).](/cms/asset/0c9b61cd-ff6a-4a5f-8626-7ecb30946778/ianb_a_1544142_f0005_c.jpg)
Figure 6. Cytotoxicity of Bio-PA NPs (a), EPI(b) and Bio-PA/EPI NPs (c) against HepG2 cells in 2D and 3D culture model.
![Figure 6. Cytotoxicity of Bio-PA NPs (a), EPI(b) and Bio-PA/EPI NPs (c) against HepG2 cells in 2D and 3D culture model.](/cms/asset/fcaefa47-f5b6-489f-9d93-db9678a4262d/ianb_a_1544142_f0006_c.jpg)
Table 1. IC50 of HepG2 Cells after 24, 48 and 72-h incubation with EPI, Bio-PA NPs at various drug concentrations in 2D and 3D culture method.
Figure 7. The changes of the tumor volumes in the tumor-bearing mice after 2D and 3D HepG2 cells subcutaneous injection (a). Average weights of different groups in the process of tumor growth (b). The picture of tumors/hydrogels removed from nude mice at specific time intervals after inoculated subcutaneously (c). Representative photomicrographs of the tumor sections (H&E staining) of mice of 2D and 3D (d).
![Figure 7. The changes of the tumor volumes in the tumor-bearing mice after 2D and 3D HepG2 cells subcutaneous injection (a). Average weights of different groups in the process of tumor growth (b). The picture of tumors/hydrogels removed from nude mice at specific time intervals after inoculated subcutaneously (c). Representative photomicrographs of the tumor sections (H&E staining) of mice of 2D and 3D (d).](/cms/asset/c6bd2543-eaf3-441f-a7f1-a0a49878802a/ianb_a_1544142_f0007_c.jpg)