Advanced search
Publication Cover
Artificial Cells, Nanomedicine, and Biotechnology
An International Journal
Volume 47, 2019 - Issue 1
Submit an article Journal homepage
2,212
Views
17
CrossRef citations to date
0
Altmetric
Research Article

Micro/nanostructured TiO2/ZnO coating enhances osteogenic activity of SaOS-2 cells

Ranran Zhanga State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, China;b Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China;c Key Laboratory of Advanced Materials of Ministry of Education of China, Tsinghua University, Beijing, ChinaView further author information
,
Nan Xua State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, China;c Key Laboratory of Advanced Materials of Ministry of Education of China, Tsinghua University, Beijing, ChinaView further author information
,
Xujie Liua State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, China;b Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China;c Key Laboratory of Advanced Materials of Ministry of Education of China, Tsinghua University, Beijing, ChinaView further author information
,
Xing Yanga State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, China;c Key Laboratory of Advanced Materials of Ministry of Education of China, Tsinghua University, Beijing, ChinaView further author information
,
Hao Yana State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, China;b Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China;c Key Laboratory of Advanced Materials of Ministry of Education of China, Tsinghua University, Beijing, ChinaView further author information
,
Jing Maa State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, ChinaView further author information
,
Qingling Fenga State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, China;c Key Laboratory of Advanced Materials of Ministry of Education of China, Tsinghua University, Beijing, ChinaCorrespondence[email protected]
View further author information
&
Zhijian Shena State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, China;d Department of Materials and Environmental Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, SwedenCorrespondence[email protected] [email protected]
View further author information
 show all
Pages 2838-2845 | Received 18 Aug 2018, Accepted 29 Oct 2018, Published online: 15 Jul 2019

Figures & data

Figure 1. SEM characterization of MAO (a–c) and MHTZn (d–f) morphology.

Figure 1. SEM characterization of MAO (a–c) and MHTZn (d–f) morphology.

Figure 2. Contact angles of MAO and MHTZn coatings. Values represent mean ± SD (n = 3). * depicts statistical differences. **p < .01.

Figure 2. Contact angles of MAO and MHTZn coatings. Values represent mean ± SD (n = 3). * depicts statistical differences. **p < .01.

Figure 3. Fibronectin adsorption of MAO (a) and MHTZn (b) groups and quantitative analysis (c). Values represent mean ± SD (n = 3). * depicts statistical differences. **p < .01.

Figure 3. Fibronectin adsorption of MAO (a) and MHTZn (b) groups and quantitative analysis (c). Values represent mean ± SD (n = 3). * depicts statistical differences. **p < .01.

Figure 4. Cytoskeletal and nuclear staining (a) and quantitative analysis (b) of SaOS-2 cells attached to MAO and MHTZn coatings at 4 and 24 h. Values represent mean ± SD (n = 3).

Figure 4. Cytoskeletal and nuclear staining (a) and quantitative analysis (b) of SaOS-2 cells attached to MAO and MHTZn coatings at 4 and 24 h. Values represent mean ± SD (n = 3).

Figure 5. Morphologies of attached cells on the MAO at 4 h (a) and insert, 24 h (c) and insert; MHTZn at 4 h (b) and insert, 24 h (d) and insert.

Figure 5. Morphologies of attached cells on the MAO at 4 h (a) and insert, 24 h (c) and insert; MHTZn at 4 h (b) and insert, 24 h (d) and insert.

Figure 6. ALP activity (a) and collagen secretion (b) of SaOS-2 cells cultured on MAO and MHTZn samples for 7 days. Values represent mean ± SD (n = 3). * depicts statistical differences. **p < .01.

Figure 6. ALP activity (a) and collagen secretion (b) of SaOS-2 cells cultured on MAO and MHTZn samples for 7 days. Values represent mean ± SD (n = 3). * depicts statistical differences. **p < .01.

Figure 7. Immunofluorescence detection of SaOS-2 cells grow on the MAO coating: OPN (a), Col-I (b), and OCN (c); and the MHTZn coating: OPN (d), Col-I (e), and OCN (f) for 14 days.

Figure 7. Immunofluorescence detection of SaOS-2 cells grow on the MAO coating: OPN (a), Col-I (b), and OCN (c); and the MHTZn coating: OPN (d), Col-I (e), and OCN (f) for 14 days.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.