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Research Article

Anticancer activity of green synthesised gold nanoparticles from Marsdenia tenacissima inhibits A549 cell proliferation through the apoptotic pathway

ORCID Icon, , , , &
Pages 4012-4019 | Received 11 Dec 2018, Accepted 29 Dec 2018, Published online: 08 Oct 2019

Figures & data

Figure 1. (a) The synthesis of AuNPs from aqueous leaf extract of Marsdenia tenacissima was confirmed by changes in solution nature from light color to dark color. (b). UV-visible absorption spectrum of synthesized AuNPs.

Figure 1. (a) The synthesis of AuNPs from aqueous leaf extract of Marsdenia tenacissima was confirmed by changes in solution nature from light color to dark color. (b). UV-visible absorption spectrum of synthesized AuNPs.

Figure 2. (a) High resolution transmission electron microscopy (HR-TEM) and (b) Energy dispersive X-ray (EDX) and analysis silver nanoparticles synthesised from Marsdenia tenacissima.

Figure 2. (a) High resolution transmission electron microscopy (HR-TEM) and (b) Energy dispersive X-ray (EDX) and analysis silver nanoparticles synthesised from Marsdenia tenacissima.

Figure 3. Fourier transforms infrared (FTIR) spectroscopy analysis of gold nanoparticles (AuNPs) synthesized from Marsdenia tenacissima.

Figure 3. Fourier transforms infrared (FTIR) spectroscopy analysis of gold nanoparticles (AuNPs) synthesized from Marsdenia tenacissima.

Figure 4. SAED pattern analysis of gold nanoparticles synthesised from Marsdenia tenacissima.

Figure 4. SAED pattern analysis of gold nanoparticles synthesised from Marsdenia tenacissima.

Figure 5. Atomic force microscopyanalysis of gold nanoparticles synthesised from Marsdenia tenacissima.

Figure 5. Atomic force microscopyanalysis of gold nanoparticles synthesised from Marsdenia tenacissima.

Figure 6. Cytotoxic potential of gold from Marsdenia tenacissima in prostate cancer A549 cell.

Figure 6. Cytotoxic potential of gold from Marsdenia tenacissima in prostate cancer A549 cell.

Figure 7. AuNPs mediated apoptotic morphological studied by AO/PI staining.

Figure 7. AuNPs mediated apoptotic morphological studied by AO/PI staining.

Figure 8. (a-c) Colorimetric assay for caspase-8,9 and 3 activity expression in AuNPs treated A549 cells. (d). Western blotting images for Bax, Bid, and Bcl-2 protein expression in AuNPs treated A549 cells.

Figure 8. (a-c) Colorimetric assay for caspase-8,9 and 3 activity expression in AuNPs treated A549 cells. (d). Western blotting images for Bax, Bid, and Bcl-2 protein expression in AuNPs treated A549 cells.