Magnetite nanoparticle as a support for stabilization of chondroitinase ABCI

Figures & data

Figure 1. Schematic representation of magnetite synthesis and cABCI immobilization.

Figure 2. The analyses of (a) XRD; (b) VSM; and (c) SEM for pristine Fe₃O₄ nanoparticle.

Figure 3. (a) FTIR spectra for pristine Fe₃O₄ nanoparticle, free cABCI enzyme and Fe₃O₄@cABCI. Influences of (b) solution pH; (c) incubation temperature; (d) cABCI-to-Fe₃O₄ mass ratio; and (e) incubation time of cABCI and Fe₃O₄ particles on immobilization efficiency of chondroitinase ABCI enzyme.

Figure 4. Zeta potential of Fe₃O₄ nanoparticle versus solution pH.

Figure 5. (a) The Michaelis–Menten plot; (b) influence of pH; (c) pH stability; (d) thermal stability; (e) storage stability at –20 °C; (f) storage stability at 4 °C; and (g) storage stability at 37 °C for free (—) and immobilized (––) cABCI enzyme. The activity was measured in 50 mM phosphate buffer at 25 °C and pH 8.

Table 1. Kinetic parameters for free and immobilized cABCI.

Sample	Vmax (µmol/min)	Km (mg/mL)	Specific activity (U/mg)
Free cABCI	0.037	1.84	46 ± 3.68
Immobilized cABCI	0.037	2.90	41 ± 0.46

Figure 6. In vitro release of cABCI from Fe₃O₄ particles in phosphate buffer at 37 °C and pH 7.5.