Association between NOS3 polymorphisms and osteonecrosis of the femoral head

Figures & data

Table 1. Primer information for the rs1799983 and rs3918181 polymorphisms.

SNP	Primer sequence	Tm (°C)	Restriction enzyme	Restriction fragment
rs1799983	F: 5′-CATGAGGCTCAGCCCCAGAA-3′	60	SduI	TT: 206 bp; GG: 124 bp, 82bp; GT: 206 bp, 124bp, 82 bp
	R: 5′-AGTCAATCCCTTTGGTGCTCAC-3′
rs3918181	F: 5′-CAACAGTGCAGGTGAATCTCA-3′	60	RsaI	AA: 216 bp; GG: 150 bp, 66 bp; AG: 216 bp, 150bp, 66bp
	R: 5′-CAAACATTACCCGCATCCT-3′

Table 2. Clinical information.

Clinical feature	Controls n = 90 (%)	Total cases n = 88 (%)	Non-traumatic cases n = 55 (%)	Traumatic cases n = 33 (%)
Age (year)	53.41 ± 5.12	52.36 ± 4.96	51.25 ± 3.15	55.25 ± 5.24
Gender (male/female)	49/41	48/40	30/25	18/15
Smoking	23(25.56)	30 (34.09)	21 (38.18)	9 (27.27)
Diabetes	–	11 (12.50)	6 (10.91)	5 (15.15)
Coronary heart disease	–	3 (3.41)	2 (3.64)	1 (3.03)
Thrombus	–	2 (2.27)	1 (1.82)	1 (3.03)
Hypertension	–	15 (17.05)	9 (16.36)	6 (18.18)
Hyperlipidemia	–	2 (2.27)	1 (1.82)	1 (3.03)

p > .05 both in non-traumatic and traumatic ONFH groups.

Table 3. Allele frequencies of NOS3 rs1799983 and rs3918181 polymorphisms.

Allele	Controls 2n = 180 (%)	Non-traumatic cases 2n = 110 (%)	Traumatic cases 2n = 66 (%)	General cases 2n = 176 (%)
rs1799983
G	151 (83.9)	102 (92.7)	58 (87.9)	160 (90.9)
T	29 (16.1)	8 (7.3)^a	8 (12.1)	16 (9.1)^b
rs3918181
A	55 (30.6)	41 (37.3)	22 (33.3)	63 (35.8)
G	125 (69.4)	69 (62.7)	44 (66.7)	113 (64.2)

a: χ² = 4.792, p = .029, OR = 0.408, 95%CI = 0.179–0.929; b: χ² = 3.972, p = .046, OR = 0.521, 95%CI = 0.272–0.997.

Table 4. Genotype frequencies of NOS3 rs1799983 and rs3918181 polymorphisms.

Genotype	Controls n = 90 (%)	Non-traumatic cases n = 55 (%)	Traumatic cases n = 33 (%)	Total cases n = 88 (%)
rs1799983
GG	65 (72.2)	48 (87.3)	25 (75.8)	73 (83.0)
GT	21 (23.3)	6 (10.9)	8 (24.2)	14 (15.9)
TT	4 (4.4)	1 (1.8)	0 (0.0)	1 (1.1)
rs3918181
GG	47 (52.2)	24 (43.6)	16 (48.4)	40 (45.5)
GA	31 (34.4)	21 (38.2)	12 (36.4)	33 (37.5)
AA	12 (13.3)	10 (18.2)	5 (15.2)	15 (17.0)