Figures & data
Figure 1. Expression of CXCR3 and miR-16-5p in LPS-induced A549 cell injury. (A) The effect of LPS on expression of miR-16-5p in A549 cell; (B) The effect of LPS on mRNA expression of CXCR3 in A549 cell; (C,D) The effect of LPS on protein expression of CXCR3 in A549 cell *p < .05.
Figure 2. Effects of miR-16-5p overexpression on the expression of IL-6 and TNF-α in A549 cells treated with LPS. (A) The expression level of miR-16-5p in A549 cells after overexpression miR-16-5p; (B) The effect of overexpression miR-16-5p on the mRNA expression of IL-6 and TNF-α in A549 cells treated with LPS; (C,D) Overexpression of miR-16-5p on the protein expression of IL-6 and TNF-α in A549 cells treated with LPS, compared with Con group, *p < .05; compared with LPS + miR-NC group, #p < .05.
Figure 3. Effects of miR-16-5p overexpression on apoptosis of A549 cells treated with LPS, compared with Con group. *p < .05; compared with LPS + miR-NC group, #p < .05.
Figure 4. Effect of knockdown of CXCR3 expression on LPS-induced A549 cell injury. (A) Knockdown of CXCR3 on protein expression of CXCR3 in A549 cells; (B) knockdown of CXCR3 on apoptosis of LPS-induced A549 cells; (C,D) knockdown of CXCR3 on protein expression of CXCR3, IL-6, TNF-α in LPS-induced A549 cells, compared with Con group, *p < .05; compared with LPS + si-NC group, #p < .05.
Figure 5. miR-16-5p targets CXCR3. (A) The 3'UTR of CXCR3 contains a nucleotide sequence complementary to miR-16-5p; (B) the effect of miR-16-5p on the fluorescence activity of lung cancer cells; (C,D) miR-16-5p regulates the protein expression of CXCR3, compared with miR-NC group, *p < .05; compared with anti-miR-NC group, #p < .05.
Figure 6. Overexpression of CXCR3 reverses the protective effect of miR-16-5p on LPS-induced A549 cell. (A,B) Overexpression of CXCR3 on the protein expression of IL-6, TNF-α in LPS-induced A549 cells; (C) Overexpression of CXCR3 on the apoptosis of LPS-induced A549 cells compared with LPS + miR-NC group, *p < .05; compared with LPS + miR-16-5p + pcDNA3.1 group, #p < .05.
