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Research Article

Gold nano particles synthesized from Strychni semen and its anticancer activity in cholangiocarcinoma cell (KMCH-1)

, , , , , & show all
Pages 1610-1616 | Received 17 Jan 2019, Accepted 07 Mar 2019, Published online: 10 May 2019

Figures & data

Figure 1. UV–visible spectrum absorption pattern of gold nanoparticles synthesised from Strychni semen.

Figure 1. UV–visible spectrum absorption pattern of gold nanoparticles synthesised from Strychni semen.

Figure 2. HR-Transmission electron microscopy (TEM) & Energy dispersive X-ray analysis (EDX) of gold nanoparticles synthesised from Strychni semen.

Figure 2. HR-Transmission electron microscopy (TEM) & Energy dispersive X-ray analysis (EDX) of gold nanoparticles synthesised from Strychni semen.

Figure 3. Fourier-transform infrared spectroscopy analysis and SAED pattern of gold nanoparticles synthesised from Strychni semen.

Figure 3. Fourier-transform infrared spectroscopy analysis and SAED pattern of gold nanoparticles synthesised from Strychni semen.

Figure 4. Atomic force microscopyanalysis of gold nanoparticles synthesised from Strychni semen.

Figure 4. Atomic force microscopyanalysis of gold nanoparticles synthesised from Strychni semen.

Figure 5. Cytotoxicity effect of gold nanoparticles synthesised from Strychni semen in cholangiocarcinoma cell (KMCH-1). The number of viable cells after treatment is expressed as a percentage of the vehicle-only control. This experiment was repeated thrice and the bars in the graph represent S.E. (*p < .05).

Figure 5. Cytotoxicity effect of gold nanoparticles synthesised from Strychni semen in cholangiocarcinoma cell (KMCH-1). The number of viable cells after treatment is expressed as a percentage of the vehicle-only control. This experiment was repeated thrice and the bars in the graph represent S.E. (*p < .05).

Figure 6. Apoptotic effect of gold nanoparticles synthesised from Strychni semen in KMCH-1 Cells.

Figure 6. Apoptotic effect of gold nanoparticles synthesised from Strychni semen in KMCH-1 Cells.

Figure 7. Intracellular production of ROS levels in KMCH-1 cells. This experiment was repeated thrice and the bars in the graph represent S.E. (*p < .05).

Figure 7. Intracellular production of ROS levels in KMCH-1 cells. This experiment was repeated thrice and the bars in the graph represent S.E. (*p < .05).

Figure 8. Caspase-3, Caspase-9 activity in KMCH-1 cells. This experiment was repeated thrice and the bars in the graph represent S.E. (*p < .05, #p < .01).

Figure 8. Caspase-3, Caspase-9 activity in KMCH-1 cells. This experiment was repeated thrice and the bars in the graph represent S.E. (*p < .05, #p < .01).

Figure 9. Anticancer effect of gold nanoparticles synthesised from Strychni semen on apoptotic gene expression in KMCH-1 Cells. This experiment was repeated thrice and the bars in the graph represent S.E. (*p < .05, #p < .01).

Figure 9. Anticancer effect of gold nanoparticles synthesised from Strychni semen on apoptotic gene expression in KMCH-1 Cells. This experiment was repeated thrice and the bars in the graph represent S.E. (*p < .05, #p < .01).