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Research Article

Mechanism of miR-148b inhibiting cell proliferation and migration of Schwann cells by regulating CALR

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Pages 1978-1983 | Received 08 Mar 2019, Accepted 12 Apr 2019, Published online: 07 Jun 2019

Figures & data

Figure 1. Schwann cells interfering with miR-148b. Compared with miR-con group, *P < .05; compared with anti-miR-con group, #P < .05.

Figure 1. Schwann cells interfering with miR-148b. Compared with miR-con group, *P < .05; compared with anti-miR-con group, #P < .05.

Figure 2. Effects of miR-148b intervention on Schwann cell activity. Compared with miR-con group, *P < .05; compared with anti-miR-con group, #P < .05.

Figure 2. Effects of miR-148b intervention on Schwann cell activity. Compared with miR-con group, *P < .05; compared with anti-miR-con group, #P < .05.

Figure 3. Effects of miR-148b intervention on Schwann cell migration. Compared with miR-con group, *P < .05; compared with anti-miR-con group, #P < .05.

Figure 3. Effects of miR-148b intervention on Schwann cell migration. Compared with miR-con group, *P < .05; compared with anti-miR-con group, #P < .05.

Figure 4. MiR-148b targeting CALR. (A) miR-148b targeting combined with the sequence information of the CALR mRNA 3’UTR; (B) the effect of miR-148b on the activity of Schwann cells luciferase; (C) the effect of miR-148b on the expression of CALR protein in Schwann cells; Compared with the miR-con group, *P < .05; compared with the anti-miR-con group, #P < .05.

Figure 4. MiR-148b targeting CALR. (A) miR-148b targeting combined with the sequence information of the CALR mRNA 3’UTR; (B) the effect of miR-148b on the activity of Schwann cells luciferase; (C) the effect of miR-148b on the expression of CALR protein in Schwann cells; Compared with the miR-con group, *P < .05; compared with the anti-miR-con group, #P < .05.

Figure 5. Intervention of CALR affects proliferation and migration of Schwann cells. (A) Effect of intervention of CALR on the expression of CALR protein in Schwann cells; (B) Effect of intervention of CALR on Schwann cell activity; (C) Effect of intervention of CALR on migration in Schwann cells; Compared with si-con group, *P < .05; compared with the Ctrl group, #P < .05.

Figure 5. Intervention of CALR affects proliferation and migration of Schwann cells. (A) Effect of intervention of CALR on the expression of CALR protein in Schwann cells; (B) Effect of intervention of CALR on Schwann cell activity; (C) Effect of intervention of CALR on migration in Schwann cells; Compared with si-con group, *P < .05; compared with the Ctrl group, #P < .05.

Figure 6. Effects of intervention of miR-148b and CALR on proliferation and migration of Schwann cells. (A) Effect of intervention of miR-148b and CALR on the expression of Schwann cell CALR protein; (B) Effect of intervention of miR-148b and CALR on Schwann cell activity; (C) Effect of intervention of miR-148b and CALR on Schwann cell migration; Compared with the anti-miR-con group, *P < .05; compared with the anti-miR-con + si-con group, #P < .05.

Figure 6. Effects of intervention of miR-148b and CALR on proliferation and migration of Schwann cells. (A) Effect of intervention of miR-148b and CALR on the expression of Schwann cell CALR protein; (B) Effect of intervention of miR-148b and CALR on Schwann cell activity; (C) Effect of intervention of miR-148b and CALR on Schwann cell migration; Compared with the anti-miR-con group, *P < .05; compared with the anti-miR-con + si-con group, #P < .05.