Study of As₂O₃ regulating proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1

Figures & data

Figure 1. Effects of As₂O₃ with different concentrations on proliferation of Tca8113 cells.

Figure 2. As₂O₃ inhibits Id-1 expression in Tca8113 cells compared with the control group. *p < .05.

Figure 3. Detection of Id-1 gene silencing efficiency in Tca8113 cells. A: Id-1 mRNA expression in cells; B: Id-1 protein expression in cells compared with the si-con group. *p < .05.

Figure 4. Silencing Id-1 inhibits proliferation and induces apoptosis in Tca8113 cells. A: Silencing Id-1 inhibits the proliferation of Tca8113 cells; B: Statistics of apoptotic rate of Tca8113 cells after silencing Id-1; C: Detection of apoptotic rate of Tca8113 cells compared with si-con group. *p < .05.

Figure 5. Overexpression of Id-1 partially restored the inhibition of As₂O₃ on proliferation and the promotion of apoptosis in Tca8113 cells. Note: A:qRT-PCR analyzed the expression of ld-1 mRNA of Tca8113 cells. B: Western blot determined the expression of ld-1 protein of Tca8113 cells. C: MTT assay determined the proliferation of Tca8113 cells. D:Cell apoptotic rate was detected using a flow cytometry of Tca8113 cells. *p < .05.

Figure 6. Id-1 ectopic expression partially restored the effect of As₂O₃ on the expression of apoptotic proteins in Tca8113 cells. *p < .05.