Figures & data
Figure 1. Biological features of linc00858. (A) The expression of linc00858 was upregulated in lung cancer tissues. (B) Multi-tissue analysis revealed that linc00858 mainly expressed in lung tissue. (C) The expression of linc00858 was upregulated in lung cancer cell lines.
Figure 2. Function analysis of linc00858. (A) The efficiency of overexpression was verified via qPCR. (B) Overexpression of linc00858 significantly promoted the proliferation rates in A549 and H1299 cell lines. (C) Overexpression of linc00858 significantly facilitated the EMT process through western blotting. (D) Overexpression of linc00858 significantly facilitated the EMT process through qPCR. (E) Transwell assay demonstrated that overexpression of linc00858 significantly promoted the cell invasion in A549 and H1299 cell lines.
Figure 3. miR-3182 is the direct target of linc00858. (A) The potential binding sequences were showed. (B) Dual-luciferase activity assay revealed that miR-3182 is the direct target of linc00858. (C) The expression of miR-3182 was downregulated in the linc00858 overexpression group. (D) Overexpression of miR-3182 significantly inhibited the proliferation rates in A549 and H1299 cell lines. (E) Overexpression of miR-3182 significantly inhibited the EMT process through western blotting. (F) Overexpression of miR-3182 significantly reversed the EMT process through qPCR. (G) Overexpression of miR-3182 significantly inhibited the cell invasion in A549 and H1299 cell lines.
Figure 4. linc00858 promote lung cancer development through negatively regulate miR-3182. (A) The expression of miR-3182 was significantly downregulated in lung cancer cell lines. (B) Overexpression of miR-3182 partially rescued the effect of linc00858 in A549 and H1299 cell lines. (C) Overexpression of miR-3182 can upregulated the expression of EMT markers compared with linc00858 group. (D) Rescue experiment revealed that miR-3182 promoted the cell invasion compared with linc00858 group.
Figure 5. MMP2 is the direct target of miR-3182. (A) The potential binding sequences were showed. (B) Dual-luciferase activity assay revealed that MMP2 is the direct target of miR-3182. (C) Overexpression of miR-3182 could inhibited the expression of MMP2 via qPCR. (D) Overexpression of miR-3182 could inhibited the expression of MMP2 via western blotting. (E) Overexpression of linc00858 could upregulated the expression of MMP2 via qPCR. (F) Overexpression of linc00858 could upregulated the expression of MMP2 via western blotting.
Figure 6. linc00858/miR-3182/MMP2 axis in lung cancer. (A) Overexpression of MMP2 can reversed the proliferation effect of linc00858 and miR-3182 in A549 and H1299 cell lines. (B) Overexpression of MMP2 can reversed the invasion effect of linc00858 and miR-3182 in A549 and H1299 cell lines. (C) Overexpression of MMP2 can reversed the EMT process of linc00858 and miR-3182 in A549 and H1299 cell lines via qPCR. (D) Overexpression of MMP2 can reversed the EMT process of linc00858 and miR-3182 in A549 and H1299 cell lines via western blotting.
