Figures & data
Figure 1. UV–visible spectrum absorption pattern and selected area diffraction (SAED) pattern of gold nanoparticles synthesized from R. rubescens (RR-AuNP). Nanoparticles were recorded for at different time intervals for the confirmation and detection of RR-AuNP size.
Figure 2. (A) HR-Transmission electron microscopy images illustrating the gold nanoparticle synthesized. Scale bars: 100 nm; 50 nm. (B) Energy dispersive X-ray spectrum of gold nanoparticles synthesized from R. rubescens (RRAuNP).
Figure 3. Fourier-transform infrared spectroscopy analysis (A) and dynamic light scattering (B) of gold nanoparticles synthesized from R. rubescens (RRAuNP) were assessed to elucidate the structure of RR-AuNP.
Figure 4. Atomic force microscopy analysis of gold nanoparticles synthesized from R. rubescens (RRAuNP).
Figure 5. Cytotoxicity effect of gold nanoparticles synthesised from R. rubescens (RR-AuNP) against lung carcinoma cell line A549 and Human lung fibroblast cells MRC-5 cells treated with different doses of RRAuNP. (A) Human lung fibroblast cells MRC-5 cells; (B) lung carcinoma cell line A549. Each bar represents mean ± SEM of three independent observations. Significant at *p < .05.
Figure 6. Apoptotic changes induced by the treatment of RR-AuNP were evidenced with TUNEL assay, DAPI staining and Immunoblotting analysis. (A) Apoptotic morphological changes; (B) capases activity; (C) apoptotic signalling proteins.
Figure 7. Effect of gold nanoparticles synthesized from R. rubescens (RR-AuNP) on intracellular ROS production in A549 lung carcinoma cell line. Each bar represents mean ± SEM of three independent observations. Significant at *p < .05.
