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Research Article

Silver/silver chloride (Ag/AgCl) nanoparticles synthesized from Azadirachta indica lalex and its antibiofilm activity against fluconazole resistant Candida tropicalis

Pages 2107-2113 | Received 08 Apr 2019, Accepted 08 May 2019, Published online: 29 May 2019

Figures & data

Table 1. Antibiogram of clinical isolates of Candida tropicalis.

Figure 1. (A) 1.0 mM silver nitrate solution, (B) synthesized AgNPs after 10 min.

Figure 1. (A) 1.0 mM silver nitrate solution, (B) synthesized AgNPs after 10 min.

Figure 2. Optimization of AgNPs synthesis; constant of 1.0 mM AgNO3 (5.0 ml) with different volume (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.25 and 1.5 ml) of diluted neem latex.

Figure 2. Optimization of AgNPs synthesis; constant of 1.0 mM AgNO3 (5.0 ml) with different volume (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.25 and 1.5 ml) of diluted neem latex.

Figure 3. FTIR- spectra analyses of crude neem latex (A) and synthesized AgNPs (B).

Figure 3. FTIR- spectra analyses of crude neem latex (A) and synthesized AgNPs (B).

Figure 4. XRD pattern of silver chloride (AgCl) and silver (Ag) nanoparticles.

Figure 4. XRD pattern of silver chloride (AgCl) and silver (Ag) nanoparticles.

Figure 5. SEM images of silver nanoparticles.

Figure 5. SEM images of silver nanoparticles.

Figure 6. TEM images of silver nanoparticles. (Bar: 100 nm).

Figure 6. TEM images of silver nanoparticles. (Bar: 100 nm).

Figure 7. Biofilm quantification of Candida tropicalis by XTT reduction assay.

Figure 7. Biofilm quantification of Candida tropicalis by XTT reduction assay.

Figure 8. Biofilm quantification of Candida tropicalis by ATPase assay.

Figure 8. Biofilm quantification of Candida tropicalis by ATPase assay.